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Course Name
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Molecular Biology
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Course Code
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16120130
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Weekly Hours
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1.5-1.0
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Credits
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2
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Offered Semester
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the first semestor of the third year
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Textbooks
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Instant Notes in Molecular Biology（Second Edition）,Turner P.C. et al, BIOS scientific publishers limited, 2000
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Prerequisites
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botany, zoology, biology or cytology
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Course Description
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The contents include: The concept and development of molecular biology; Subcellular organelles; The Structure, properties and analysis of proteins, nucleic acids, and genome; the molecular machnism of DNA replication, transcription and translation; DNA damage, repair and recombination; Gene regulation in prokaryotes and eukaryotes; gene Manipulation et al.
The Experiments include extraction and purification of plasmid DNA; DNA digestion with restrictiction enzymes; extraction of plant or bacterial DNA; PCR; Preparation of competent E .coli; Ligation, transformation and screening of recombined plasmid DNA et al.
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Teaching Goal and Basic Requirements:
The teaching goal of course is for the students to learn the basic theory, concepts and experimental technologies in Molecular Biology, which will make stuents have the academic and practical foundation in research, teaching and development and production in future.
During the teaching, attention shoud be paid for the linkage and distinguish between the Molecular Biology and Biochemistry or other relative courses. In class, the teaching will focus on the basic theory and principle of experiments; In expeiments, the teaching will emphasize the improvement of students’ ability on manipulation skill, analysis and resolvent of problem.
Content of Courses & Hours Allocation
Chapter 1 The concept and development of molecular biology 1 class hours
1. The Development of Molecular Biology
2. The Center Dogma
3. What’s Molecular Biology
Chapter 2 Subcellular organelles and Macromolecules 5 class hours
1. The structural difference in prokaryotic cells and eukaryotic cells
2. The Structure, Properties and Analysis of Proteins.
3. The Structure, Properties and Analysis of Nucleic Acids
4. The Structure, Properties of Genome
Chapter 3. DNA replication, transcription and translation 6 class hours
1. DNA replication
2. Transcription
3. Translation
Chapter 4 DNA damage, repair and recombination 3 class hours
1. Mutagenesis
2. DNA Damage
3. DNA Repair
4. Recombination
Chapter 5. Regulation of gene activity 3 class hours
1. Introduction
2. Gene regulation in prokaryotes
3. Gene regulation in Eukaryotes
Chapter 6. Gene Manipulation 5 class hours
1. Review of DNA cloning
2. Hosts and Cloning Vectors
3. Enzymes used in DNA cloning
4. Gene libraries and screening
5. Analysis and use of cloned DNA
Chapter 7. Prospect of molecular biology—genomics and Post genomics. 1 class hours.
Experiments:
1. Extraction and Purification of plasmid DNA 3 class hours
2. Digestion of plasmid DNA with Restrictiction enzymes and electrophoresis analysis
3 class hours
3．Extraction of Plant DNA 3 class hours
4．PCR and Analysis 3 class hours
5. Preparation of competent E .coli 3 class hours
6. Ligation, transformation and screening of recombined plasmid DNA 3 class hours
Teaching Plan:
The course is divided into class theory lectures and experiments. The principle of experiments will be explained in the class. The experiments will be a continuum,and some contents will be done in a cross way.
Recommended Textbooks and Other References:
Textbook
Instant Notes in Molecular Biology（Second Edition）,Turner P.C. et al, BIOS scientific publishers limited, 2000
References
1、Modern Molecular Biology（Second Edition）. Zhu Yuxian and Li Yi. High education publishers. 2002 (in Chinese).
2、Molecular Genetics. Sun Naien, Sun Dongxu and Zhu Dexi. Nanjing university publishers. 1995 (in Chinese).
3、Basic Molecular Biology.Yang Qisheng Nanjing university publishers. 1994 (in Chinese).
4、Molecular Biology Yan Longfei and Zhang Yulin Beijing agric. university publishers. 1993 (in Chinese).
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Course Name
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Greenhouse Management
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Course Code
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16120260
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Weekly Hours
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2.0-1.0
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Credits
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2.5
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Offered Semester
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summer
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Textbooks
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Greenhouse Operation and Management (Sixth Edition)
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Prerequisites
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Botany
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Course Description
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This course is designed to provide students with a working knowledge of current principles and practices that are important for successful greenhouse operation and management. This course will provide a foundation of basic knowledge about cultural production practices, which are required for commercial plant production in greenhouses. The environmental factors manipulated within a greenhouse and how greenhouses are designed to control these factors will be introduced in this course. Greenhouses are used to produce a variety of crops such as potted flowering plants, foliage plants, vegetables，fruits and cut flowers. Several types of cropping systems may be used in a greenhouse. The topics of greenhouse design, heating and cooling, benching, lighting, irrigation and fertilization systems, and greenhouse maintenance will also be covered in this course.
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Teaching Goal and Basic Requirements:
It’s a main and important course for horticultural students. Upon completion of this course, you will have a working knowledge of technical components of greenhouse construction, maintenance and management, understand the basic principles of plant growth in a controlled environment, have independent experience in crop production and greenhouse management decisions, and possess a collection of reference material for future use. Residential and commercial greenhouse management, practices, and problems are covered
Students are expected to learn the theoretical and applied practices of managing a greenhouse facility, and Learn the principles greenhouse management for the production of fruit, floral and vegetable crops
Content of Courses & Hours Allocation:
Unit 1 Introduction：The development future of greenhouse 2 hours
Unit 2 Structure and environmental factors 2 hours
Greenhouse Benching and Production 2 hours
Unit 3 Lighting and controlling in Greenhouse 2 hours
Greenhouse Temperature: Heating system, Ventilation and Cooling system 4 hours
Growing Media for Horticultural crops in greenhouse 2 hours
Unit 4 Mineral Elements and Greenhouse Crop Fertilization 3 hours
Water Quality and Irrigation Systems in greenhouse 3 hours
Disease and pest management in greenhouse 2 hours
Unit 5 Examples for vegetable，fruits and flowers production in greenhouse 8 hours
Horticultural Crop Production: You are responsible for the survival, health, and success of the crops for this course. The death of a crop or damage due to neglect will significantly impact your final grade.
Teaching Plan:
Exp.1 Greenhouse Structure and construction
Exp.2 Preparation the nutrient solution and soilless culture technique in horticultural crops.
Exp.3 Detection the environment factors
Exp.4 survey on the diseases and insects of Greenhouse crops
Exp.5 Evaluation the growth of horticulture crops
Participation in all scheduled class activities is expected. Absenteeism or nonparticipation will not only affect a student’s learning the material in this course, but also will adversely affect his/her grade.
We will have 3 times homeworks. The homeworks could cover any information presented before that date.
Recommended Textbooks and Other References: (books, editors, publishing company,
publishing time)
1. Textbook：PAUL V. NELSON 《Greenhouse Operation and Management 》 (Sixth Edition)
Culinary and Hospitality Industry Publications Services， 2003.9
2. References：
《设施园艺学》，李式军，高等教育出版社。2002年8月出版。
《设施园艺学》，张福墁著，中国农业出版社。2001年版
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Course Name
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Plant Quarantine
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Course Code
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16120640
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Weekly Hours
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1.5-1
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Credits
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2
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Offered Semester
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Autumn
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Textbook
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Xie Guanlin and Yu Hong Edited, English Teaching Material.
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Prerequisites
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Plant pathology, Entomology
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Course Description
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The plant quarantine course consists of three parts. It includes the basic theories, laws, standards, measurements and risk analysis for the pest management in the first part. Second part is dangerous pathogenic microorganisms and weeds, which will describe characteristics, identification, distribution, disease losses, disease cycle, detection of the microorganisms and their management. The last part is dangerous insect pests. It will introduce morphology, biology, ecology, identification, monitoring, treatment and management of the insect pest.
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Teaching Goal and Basic Requirements:
Plant Inspection and Quarantine is a core course for undergraduate students with major in plant protection. It needs students to be familiar with basic knowledge and technique in plant inspection and quarantine by the lectures and laboratory exercises and to solve the problems with knowledge learnt against dangerous pest from inside or outside China. The students are also required to understand the terms, characteristics, laws and regulations, pest risk analysis, detection and identification, monitoring and treatment methods in the quarantine pest management.
Content of Courses & Hours Allocation:
Chapter 1 An introduction to plant inspection and quarantine 2 hours
Chapter 2 Laws and regulations of plant Inspection and quarantine 2 hours
Chapter 3 Plant quarantine pests
1. Plant quarantine Coleoptera pests 3 hours
2. Lab exercise in Coleoptera pests 3 hours
3. Plant quarantine Lepidoptera pests 2 hours
4. Plant quarantine Homoptera pests 2 hours
5. Lab exercise in Lepidoptera and Homoptera pests 3 hours
6. Plant quarantine Diptera pests 2 hours
7. Others plant quarantine pests 1 hours
8. Lab exercise in Diptera and others pests 2 hours
Chapter 4 Plant Quarantine pathogens
1. Basics in Quarantine pathogens 2 hours
2. The major dangerous fungal pathogens 2 hours
3. Lab exercise I in the fungal pathogens 2 hours
4. The major dangerousfungal pathogens 1 hours
5. Lab exercise II in the fungal pathogens 2 hours
6. The major dangerous prokaryotes 2 hours
7. Lab exercise in thedangerous prokaryotes 2 hours
8. The major dangerous prokaryotes 2 hours
9. Other dangerous plant pathogens 2 hours
10. Lab exercise in otherdangerous plant pathogens 2 hours
Teaching Plan:
1. The multiple media will be used in the lectures and laboratory exercises.
2．The laboratory exercises are separated with lecture 1hours per week.
3．The survey and visiting related with plant inspection and quarantine will be organized.
4．The lecture files for discussions could be downloaded with FTP or sent by e-mail.
Recommended Textbooks and Other References:
1. Xie Guanlin and Yu Hong. Learning materials for plant inspection and quarantine (in English), 2007 (unpublished).
2. Xu Zhigang. Plant quarantine (3rd edition, in Chinese). Beijing：High education press,2007.
3. Hong Yi.Plant quarantine methods and techniques (in Chinese). Beijing：Chemistry industry press. 2005.
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Course Name
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Biostatistics and Experiment Design
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Course Code
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16121030
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Weekly Hours
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2.0-2.0
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Credits
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3.0
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Course Description
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This course mainly introduces basic principles of biostatistics, methods of experimental designs，SAS and R language programming for analyzing experimental data of common design. The main content include probability and statistical inference, experiment error control and simple data analysis, matrix theory and principle of simple regression analysis, multivariate regression analysis, other multivariate regression and nonlinear regression, variance and reandomized block design, nested design analysis, covariance analysis, mixed linear models.
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Teaching Goal and Basic Requirements:
TeachingGoal: Students are required to understand methods for common-used experimental design and data analysis, and be able to make correct statistical inference on the analysis results. After study of this course, students will have basic capability of biostatistics necessary for life science research.
Basic Requirements: Students are required to undearstand the principles of methods for experimental design and statistical analysis methods, have ability to establish statistical model according to experiment design, and analyze data using SAS software and R language programming, could understand results of statistical analysis and conduct correct statistical inference.
Content of Courses & Hours Allocation:
This course includes 40 hours teaching in classroom and 16 hours experiments in lab.
1. Probability and statistical inference 3 hours
2. Experimental error control and simple data analysis 4 hours
3. Matrix theory and principle of simple regression analysis 3 hours
4. Multivariate regression analysis 6 hours
5. Other multivariate regression and nonlinear regression 3 hours
6. Variance analysis and randomized block design 6 hours
7. Factorial design analysis 3 hours
8. Nested design analysis 3 hours
9. Covariance analysis 3 hours
10. Mixed linear models 6 hours
Experiment
1. Basic of SAS and R language programming 0 hours
2. Simple data analysis 4 hours
3. Regression analysis 4 hours
4. Variance analysis 4 hours
5. Covariance analysis and mixed model analysis 4 hours
Teaching Plan:
1. Rollcall will be employed in random on some class, which will be in charge of teaching assistant;
2. Experimental report and corresponding homework are required to submit and marks of evaluation will be included in final score;
3. One mid-examination will be arranged, the result will be included in final score, ;
4. Every student is requested to join one study group of 5-6 persons, each group is required to finish assigned taskand report in PPT slides, the evaluation will be included in final score;
5. One website will be created where study materials will be provided
Recommended Textbooks and Other References: (books, editors, publishing company, Publishing time)
1. Principles of linear model analysis, Zhu Jun, Science Press, 1999
2. Methods of Experimental Statistics, Gai Jun-yi, China Agricultural Press, 2000
3. Statistical Designs and Analysis of Experiments (2nd), Robert L. Mason, Richard F. Gunst, James L. Hess, John Wiley & Sons, Inc, 2003
4. Introduction to Biostatistics (2nd), Robert R. Sokal and F. James Rohlf. Dover Publication, INC, Mincola, New York，2008
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Course Name
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Bioinformatics
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Course Code
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16105139
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Weekly Hours
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1.5
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Credits
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1.5
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Textbooks
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《Bioinformatics: Sequence and Genome Analysis》（David Mount，Cold Spring Harbor Laboratory; ISBN: 0879696087，2001）
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Prerequisites
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Course Description
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Bioinformatics is a new discipline of obtaining information about genomic or protein sequence data. This may involve similarity searches of databases, comparing your unidentified sequence to the sequences in a database, or making predictions about the sequence based on current knowledge of similar sequences. It is an important assistance of other disciplines, such as genomics, molecular biology, molecular evolution etc. Goals of this course: (1) For students of bioinformatics major, should understand basic lines, statistics and front field of this discipline; (2) For students of non-bioinformatics major, should know main molecular databases, basic knowledge and tools of bioinformatics.
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Teaching Goal and Basic Requirements:
Bioinformatics is a new discipline of obtaining information about genomic or protein sequence data. This may involve similarity searches of databases, comparing your unidentified sequence to the sequences in a database, or making predictions about the sequence based on current knowledge of similar sequences. It is an important assistance of other disciplines, such as genomics, molecular biology, molecular evolution etc. Goals of this course: (1) For students of bioinformatics major, should understand basic lines, statistics and front field of this discipline; (2) For students of non-bioinformatics major, should know main molecular databases, basic knowledge and tools of bioinformatics.
Content of Courses & Hours Allocation:
Total 24 hours:
lecture 18 hours:
Chaper1 Introduction and Overview（2 hours）
Chaper2 QTL mapping（2 hours）
Chaper3 Microarray data analysis（2 hours）
Chaper4 Sequence Alignment（2 hours）
Chaper5 Database Searching for Similar Sequences（2 hours）
Chaper6 Gene Prediction（2 hours）
Chaper7 Genome Analysis（2 hours）
Chaper8 Protein Classification and Structure Prediction （2 hours）
Chaper9 Phylogenetic Prediction（2 hours）
Experiment 1. Construction of Genetic Maps（2 hours）
Experiment 2. Analysis of DNA Sequence （2 hours）
Experiment 3. Multiple Sequence Alignments （2 hours）
Teaching Plan:
Homework is required
Recommended Textbooks and Other References: (books, editors, publishing company,
publishing time)
《Bioinformatics: Sequence and Genome Analysis》（David Mount，Cold Spring Harbor Laboratory; ISBN: 0879696087，2001