报告人： Zhixin Xie（谢志新）教授（美国德州理工大学）
报告时间：12月19日（周四）下午3：00
报告地点：农生环大楼A435
报告主持人：樊龙江
报告人简介：
Ÿ Associate professor, Department of biological sciences, Texas Tech University
Ÿ Ph.D., Molecular Biology & Biochemistry, University of Idaho (2000)
Ÿ M.S., Plant Genetic Improvement, Zhejiang University
Ÿ B.S., Agronomy, Zhejiang University
Research in his laboratory is directed to understand how multiple small RNA pathways operate in plants using genetic, genomic, and biochemical approaches. Most eukaryotic organisms possess highly conserved RNA silencing machinery that generates 21- ~ 24-nucleotide small RNAs from double stranded RNA precursors. These small RNAs, which include microRNA (miRNA) and several classes of small interfering RNAs (siRNA), play important roles in regulating gene expression, heterochromatin formation, and defense against invading viruses. The core components of RNA silencing machinery involve several evolutionarily conserved protein families, including DICER (DCR) or DICER-LIKE (DCL), ARGONAUTE (AGO), and, in some cases, RNA-DEPENDENT RNA POLYMERASE (RDR). Interestingly, plants encode multiple DCL and RDR proteins. Recent genetic studies have revealed multiple small RNA pathways in plants; each requires a distinct set of DCL and RDR factors. Plants, therefore, provide a unique system to study the genetic diversification and functional adaptation of small RNA pathways.
Selected Publications：
· Xie Z , Khanna K, Ruan S. (2010) Expression of MicroRNAs and Its Regulation in Plants. Semin Cell Dev Biol 21 , 790-797
· Allen E, Xie Z , Gustafson AM, Carrington JC. (2005) microRNA-directed phasing during trans-acting siRNA biogenesis in plants. Cell 121 , 207-21
· Allen E, Xie Z , Gustafson AM, Sung GH, Spatafora JW, Carrington JC. (2004) Evolution of microRNA genes by inverted duplication of target gene sequences in Arabidopsis thaliana . Nat Genet 36 , 1282-90
· Chan SW, Zilberman D, Xie Z , Johansen LK, Carrington JC, Jacobsen SE. (2004) RNA silencing genes control de novo DNA methylation. Science 303 , 1336
· Kasschau KD, Xie Z , Allen E, Llave C, Chapman EJ, Krizan KA, Carrington JC. (2003) P1/HC-Pro, a viral suppressor of RNA silencing, interferes with Arabidopsis development and miRNA function. Dev Cell 4 , 205-17
· Llave C, Xie Z , Kasschau KD, Carrington JC. (2002) Cleavage of Scarecrow-like mRNA targets directed by a class of Arabidopsis miRNA. Science 297 , 2053-6
浙江大学农学系/作物研究所
2013-12-17
