报告题目：A single gene encodes the transposition functions of TED, an autonomous element of the Mutator superfamily in maize
报 告 人：Li Yubin博士（美国罗格斯大学Waksman研究所）
报告时间：2012年4月23日上午10:00
报告地点：农生环组团A座435室
联 系 人：农学系徐建红研究员（88982406）
报告摘要：
The new mutable allele bz-m175 arose in a cross between High-Loss X High-Knob, stocks that which carry transposons from different superfamilies. The transposon in bz-m175 is an autonomous member of the Mutator superfamily, which we have named TED (Transposon Ellen Dempsey). However, unlike MuDR, TED does not encode a second function (B), which has been postulated to play a role in MuDR reinsertion after excision.
To assess if the absence of a B function affected TED reinsertion, we have isolated and characterized Bz’ germinal revertants from bz-m175. We have found that 8 of 23 germinal Bz’ have a trTED element. Furthermore, among 50 stable bz-s derivatives from bz- m175, 11 had a trTED element, 29 had a defective TED (dTED) element at bz, the rest ten had an adjacent deletion without reinsertion of TED. All the trTED elements can drive transposition of dTEDs at bz, suggesting that none of the new trTED insertion sites are silenced, and that a B function is not required for TED excision or reinsertion. Sequence analysis of trTED insertion sites showed that TED preferentially targets genes, like most other DNA transposable elements, and transposes to unlinked sites, like MuDR.
