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Dr. Miao Huiying visits the University of California, Davis for academic exchange

Date:2018-07-02 Hits:134

Dr. Miao Huiying has been invited by Professor Liu Bo to visit his laboratory located in the Department of Plant Biology at the University of California in Davis, California and carry out collaborative research from March 30, 2016 to March 21, 2018. During this period, they established a cytoskeleton-based assay for protein-protein interaction (CAPPI), which could report the interactions between signaling molecules as well as between nuclear transcriptional regulators encoded by genes in Arabidopis thaliana in the leaf cells of tobacco (Nicotiana benthamiana) upon transient expression. Furthermore, they investigated the function of γTuRC protein GCP6 in microtubule nucleation in plant cells. 




Determination of specific interaction between the nucleus-localized BIN2 and BES1 in tobacco leaf cells. Scale bar, 20 mm.

(a–c) When solely expressed in either GFP or RFP fusions, BIN2, BES1, and BUB3.1 (negative control) all localize to the nucleus. (d–f) When BIN2-GFP and BES1-RFP are expressed together, both fusion proteins remain in the nucleus. (g–i) When BIN2 is fused with the F-actin-binding Lifeact motif and GFP, the Lifeact- BIN2-GFP fusion protein recruits BES1-RFP to F-actin filaments. (j–l) When BIN2 is fused with the microtubule-binding domain (MBD) and GFP, the MBD-BIN2-GFP fusion protein recruits BES1-RFP to cortical microtubules. (m–o) While the Lifeact-BIN2-GFP fusion protein decorates F-actin, the non-interacting BUB3.1 remains in the nucleus.