吴建祥 教授
Curriculum Vitae
Education:
Ph.D. 2/2004- 3/2008, Major: Plant Pathology; Institute of Biotechnology, Zhejiang University, Hangzhou, Zhejiang, China
M.S. 9/1999- 6/2002, Major: Preventive Veterinary, Department of Animal Medical Sciences, College of Animal Sciences, Zhejiang University
B.S. 9/1990-6/1994, Major: Veterinary major; Department of Animal Medical Sciences, College of Animal Sciences, Zhejiang University
Professional Experience:
Professor, 2013.1-Present, Institute of Biotechnology, Zhejiang University
Associate Professor,12/2005-12/2012, Institute of Biotechnology, Zhejiang University;
Senior visiting scholar, 06/2012-12/2012, Department of Plant and Environmental Protection Sciences, University of Hawaii, USA
Research associate,01/2000-11/2005,Institute of Biotechnology, Zhejiang University
Research assistant,08/1994-12/1999, Institute of Biotechnology, Zhejiang University
Current Position: Prof., Vice Director, Institute of Biotechnology,College of Agriculture & Biotechnology, Zhejiang University
Address:
Tel: 0086(571)88982250; E-mail: wujx@zju.edu.cn
Area of Specialisation:
Applied Immunology, Plant Virology, Plant Pathology
Prof. Jianxiang Wu works on researches of genomic functions of plant viruses, molecular interaction between plant virus and its host/vector, RNA silencing, Breeding of transgenic rice varieties resistant to rice viruses,plant bioreactors, applied immunology. He has systematically studied the genomic function of Rice stripe virus, molecular interaction mechanisms between Rice stripe virus/Rice black-streaked dwarf virus and rice host/ small brown planthopper vector, proofed that edible vaccines of Infectious bursal disease and infectious bronchitis produced with transgenic rice and potato plants were feasible. So far, he has prepared more than 100 monoclonal antibodies against viruses, bacteria, antibiotics, pesticides and toxins, and developed serological methods and their immunological kits for the detection of these substances using the prepared monoclonal antibodies. Among these kits, the plant virus detection kits were massively applied in whole China after the promotion of the Ministry of Agriculture. Cooperating with the Ministry of Agriculture, plant protection stations, and China Agriculture Research System, forecasting and early warning and real-time monitoring platforms for a variety of important plant viruses in China have been established. These platforms contribute to scientific prevention and control, decreasing pesticide use, saving labor and production costs, and have achieved remarkable economic, ecological and social benefits. He successively presides over projects of the National Natural Science Foundation of China, the National Basic Research Program (973) of China, the National High-tech R&D Program (863) of China, the Natural Science Foundation of Zhejiang Province of China, the Program of Science and Technology of Zhejiang Province, the National Key Research and Development Project of China, the Public Science and Technology Research Funds Projects of Agriculture, and so on. He has obtained one Second Class Prize of the State Scientific and Technological Progress Award, one First Prize of Scientific and Technological Progress Award of the Ministry of Education, and one First, two Second and one Third Prizes of Scientific and Technological Progress Award of the Zhejiang Province. He has already published 119 papers in Autophagy, Scientific Reports, Molecular Plant, PLoS one, Plant Biotechnology Journal, Journal of virology, Virology, Virus Research, Virology Journal, Journal of Virological Methods, and among them, there are 55 SCI papers. He also published one textbook of molecular biology experiment. Moreover, he has 39 national invention patents that have been authorized.
Grants
Wu, J.X. et al. the National Natural Science of China (31272015)‚ 2016-2019. RMB696,000
Wu, J.X. et al. The national key basic research and development program of China (2014CB138400)‚ 2014-2018. RMB1500,000
Wu, J.X. et al. the National Natural Science of China (31272015)‚ 2013-2016. RMB850,000
Wu, J.X. et al. The
Wu, J.X. et al. The special fund for agro-scientific research in the public interest from the Ministry of Agriculture, China (201303021), 2013-2017. RMB722,500
Wu, J.X. et al. The special fund for agro-scientific research in the public interest from the Ministry of Agriculture, China (201203076-05), 2012-2016. RMB1050,000
Publication:
1) Zhang MH, Chen R, Zhou XP, Wu JX*. Monoclonal antibody-based serological detection methods for Wheat dwarf virus. Virologica Sinica, 2018,33：162-172. doi.org/10.1007/s12250-018-0024-3（SCI)
2) Yu C, Yang CY, Song SY1, Yu ZX, Zhou XP, Wu JX*. Development of a sensitive Luminex Xmap-based microsphere immunoassay for specific detection of Iris yellow spot virus. Virology Journal, 2018,15:62.（SCI, IF=2.2)
3) Fu S, Xu Y, Li CY, Li Y, Wu JX, Zhou XP. Rice stripe virus interferes with S-acylation of remorin and induces its autophagic degradation to facilitate virus infection. Molecular Plant, 2018, 11, 269-287. doi: 10.1016/j.molp.2017.11.011.（SCI, IF=8.8)
4) Bin Y, Li Z, Wu J, Wang X, Zhou Y, Li T, Yang F, Zhou C, Song Z. Development of an immunochromatographic strip test for rapid detection of citrus yellow vein clearing virus. Archive of Virology. 2018,163:349-357. DOI 10.1007/s00705-017-3558-z（SCI, IF=2.1)
5) Song G, WuJY, XieY, LiuY, Qian YJ, ZhouXP, WuJX*. Monoclonal antibody-based serological assays for detection of Potato virus S in potato plants. J Zhejiang Univ-Sci B (Biomed & Biotechnol). 2017,18(12):1075-1082.（SCI, IF=1.7)
6) Rao LX, Guo YS, Zhang LL, Zhou XP, Hong J, Wu JX*. Genetic variation and population structure of Cucumber green mottle mosaic virus. Archives of virology. 2017, 162(5): 1159-1168. DOI 10.1007/s00705-016-3207-y. (*Corresponding author, SCI, IF=2.1)
7) Liu Z, Sunzhu YJ, Zhou XP, Jian H, Wu JX*. Monoclonal antibody-based serological detection of Citrus yellow vein clearing virus in citrus groves. Journal of Integrative Agriculture. 2017, 16(4):884-891. (*Corresponding author, SCI, IF=1.2)
8) Chen Z, Zhang MH, Zhou XP, Wu JX*. Development and detection application of monoclonal antibodies against Zhejiang isolate of Zucchini yellow mosaic virus. Journal of Integrative Agriculture. 2017, 16(1): 115-124. (*Corresponding author, SCI, IF=1.2)
9) Wang LL, Wang XR, Wei XM, Huang H, Wu JX, Chen XX, Liu SS, Wang XW*.The autophagy pathway participates in resistance to Tomato yellow leaf curl virus infection in whiteflies. Autophagy. 2016, 12(9):1560-1574. (SCI, IF=9.1)
10) Chen S, Jiang GZ, Wu JX, Liu Y, Qian YJ, Zhou XP. Characterization of a Novel Polerovirus Infecting Maize in China, Viruses, 2016,8(5):120(SCI, IF=3.64)
11) Liu Z, Chen Z, Hong J, Wang XF, Zhou CY, Zhou XP, Wu JX*. Monoclonal antibody-based serological methods for detecting Citrus tristeza virus in citrus groves. Virologica Sinica, 2016, 31 (4): 324-330.（*Corresponding author, SCI）
12) Li N, Chen Z, Liu Y, Liu Y, Zhou XP, Wu JX*. Development of monoclonal antibodies and serological assays specific for Barley yellow dwarf virus GAV strain. Virology Journal. 2015, online. (*Corresponding author, SCI，IF=2.2)
13) Huang HJ, Bao YY, Lao SH, Huang XH, Ye YZ, Wu JX, Xu HJ, Zhou XP. Zhang CX. Rice ragged stunt virusinduced apoptosis affects virus transmission from its insect vector, the brown planthopper to the rice plant. Scientific Reports. 2015, 5:11413. DOI: 10.1038/srep11413 (SCI，IF=5.1)
14) Huang LZ, Li ZF, Wu JX, Xu Y, Yang XL, Fan LJ, Fang RX, Zhou XP. Analysis of genetic variation and diversity of Rice stripe virus populations through high-throughput sequencing. Frontiers in Plant science. 2015, 6, 176. doi: 10.3389/fpls.2015.00176. (SCI，IF=3.6)
15) Xu Y, Wu JX, Fu S, Li C Y, Zhu ZR, Zhou XP. Rice stripe Tenuivirus nonstructural protein 3 hijacks the 26S proteasome of the small brown planthopper via direct interaction with regulatory particle non-ATPase subunit 3. Journal of Virology. 2015, 89(8):4296-4310. (SCI，IF=4.8)
16) Kong LF*, Wu JX*, Lu LN, Xu Y, Zhou XP. Interaction between Rice stripe virus disease-specific protein and host PsbP enhances virus symptoms. Molecular Plant. 2014,7(4):691-708. (*These authors contribute equally to this paper, SCI，IF=8.8)
17) Liu H, Song XJ, Ni YQ, Lu LN, Zhou XP, Wu JX*. Highly sensitive and specific monoclonal antibody-based serological methods for Rice ragged stunt virus detection in rice plants and rice brown planthopper vectors. Journal of Integrative Agriculture. Advanced Online Publication: 2013, Doi: 10.1016/S2095-3119(13)60533-X. （*Corresponding author, SCI, IF=1.2)
18) Wang Q, Zhou XP, Wu JX*. First Report of Maize chlorotic mottle virus Infecting Sugarcane (Saccharum officinarum). Plant Disease. 2014, 98:572.（*Corresponding author, SCI, IF=3.2)
19) Wu JX, Ni YQ, Liu H, Ding M, Zhou XP. Monoclonal antibody-based serological assays and immunocapture-RT-PCR for detecting Rice dwarf virus in field rice plants and leafhopper vectors.Journal of Virological Methods. 2014, 195:134-140. (SCI, IF=2.0)
20) Cheng ZB, Li S, Gao RZ, Sun F, Liu WC, Zhou GH, Wu JX, Zhou XP, Zhou YJ.Distribution and genetic diversity of Southern rice black-streaked dwarf virus in China.Virology Journal, 2013, 10:307. (SCI，IF=2.2)
21) Huang CJ, Hu GJ, Li FF, Li YQ, Wu JX, Zhou XP. NbPHAN, a MYB transcriptional factor, regulates leaf development and affects drought tolerance in Nicotiana benthamiana. Physiologia Plantarum. 2013, 149: 297-309. （SCI, IF=3.66)
22) Huang LZ, Rao LX, Zhou XP, Wu JX*.Genetic variability and evolution of Rice stripe virus, J Zhejiang Univ-Sci B (Biomed & Biotechnol). 2013, 14(10): 875-885. （*Corresponding author, SCI, IF=1.7)
23) Wu JX, Wang Q, Liu H, Qian YJ, Xie Y, Zhou XP. Monoclonal antibody-based serological methods for maize chlorotic mottle virus detection in China. J Zhejiang Univ-Sci B (Biomed & Biotechnol). 2013, 14(7): 555-562.（SCI, IF=1.7)
24) Xie Y, Jiao XY, Zhou XP, Liu H, Ni YQ, Wu JX*. Highly sensitive serological methods for detecting Tomato yellow leaf curl virus in tomato plants and whiteflies. Virology Journal. 2013, 10:142 (*Corresponding author, SCI，IF=2.2)
25) Wu JX, Ni YQ, Liu H, Rao LX, Zhou YJ, Zhou XP. Development and use of three monoclonal antibodies for the detection of Rice black-streaked dwarf virus in field plants and planthopper vectors.Virology Journal. 2013, 10:114. （SCI, IF=2.2)
26) Xu Y, Huang LZ, Fu S, Wu JX*, Zhou XP*. Population Diversity of Rice Stripe Virus-Derived siRNAs in Three Different Hosts and RNAi-Based Antiviral Immunity in Laodelphgax striatellus. PLoS one. 2012. 7(9): e46238. (*Corresponding author, SCI，IF=4.0)
27) Xu Y, Zhou WW, Zhou YJ, Wu JX*, Zhou XP*. Transcriptome and comparative gene expression analysis of Sogatella furcifera (Horváth) in response to Southern rice black-streaked dwarf virus. PLoS one. 2012, 7(4): e36238. (*Corresponding author, SCI，IF=4.0)
28) Wu JX, Shang HL, Xie Y, Shen QT, Zhou XP. Monoclonal antibodies against the whitefly-transmitted Tomato yellow leaf curl virus and their application in virus detection. Journal of Integrative Agriculture. 2012, 11(2): 263-268. (SCI，IF=1.2)
29) Zhang J, Dong JY, Xu Y, Wu JX*. V2 protein encoded by Tomato yellow leaf curl
30) Shang HL, Xie Y, Zhou XP, Qian YJ, Wu JX*. Monoclonal antibody-based serological methods for detection of Cucumber green mottle mosaic virus. Virology Journal. 2011, 8:228. (*Corresponding author, SCI，IF=2.2)
31) Wu JX*, Meng CM, Shang HL, Rong S, Zhang C, Hong J, Zhou XP. Monoclonal antibody-based triple antibody sandwich-enzyme-linked immunosorbent assay and immunocapture reverse transcription-polymerase chain reaction for Odontoglossum ringspot virus detection. Journal of Virological Methods, 2011,171(1) :40-45. (*Corresponding author, SCI，IF=2.0)
32) Cheng XF, Wang XQ, Wu JX,. Briddon R W, Zhou XP.βC1 encoded by tomato yellow leaf curl China betasatellite forms multimeric complexes in vitro and in vivo. Virology, 2011, 409,156–162. (SCI，IF=3.0)
33) Li W, Wu JX, Tu YY. Synergistic effects of tea polyphenols and ascorbic acid on human lung adenocarcinoma SPC-A-1 cells. Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2010, 11(6):458-464. （SCI，IF=1.7）
34) Jiang T, Liu B, Liao BR, Wu JX, Huang CJ. Malvastrum yellow vein Yunnan virus is a monopartite begomovirus，Acta Virologica，2010，54（1）：17-22（SCI，IF=0.81）
35) Wu JX, Zhang SE, Zhou XP. Monoclonal antibody-based ELISA and colloidal gold-based immunochromatographic assay for streptomycin residue detection in milk and swine urine. Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2010, 11(1):52-60. （SCI，IF=1.7）
36) Wu XY, Zh XP, Li ZH, Gao WN, Wu JX*.Monoclonal antibodies against Tobacco rattle virus and its application in the detection of TRV isolates. Indian Journal of Virology，2009, 20(2):64-69. (*Corresponding author, SCI，IF=0.3)
37) Wang D, Lu Q, Li XF, Jiang Q S, Wu JX*, Jiang DA*. Relationship between rubisco activase isoform levels and photosynthetic rate in different leaf positions of rice plant. Photosynthetica 2009, 47 (4): 621-629. (*Corresponding author, SCI，IF=1.0)
38) Xia XJ, Huang LF, Zhou YH, Mao WH, Shi K, Wu JX, Asami T, Chen ZX, Yu JQ. Brassinosteroids promote photosynthesis and growth by enhancing activation of Rubisco and expression of photosynthetic genes in Cucumis sativus, PLANTA, 2009, 230( 6), 1185-1196. (*Corresponding author, SCI，IF=3.0)
39) Wu JX, Yu C, Yang CY, Deng FL, Zhou XP. Monoclonal antibodies against the recombinant nucleocapsid protein of tomato spotted wilt virus and its application in the virus detection. Journal of Phytopathology. 2009, 157, 344-349.( IF：0.896)
40) Jin RY, Guo YR, Wang CM, Wu JX, Zhu GN. Development of a bispecific monoclonal antibody to pesticide carbofuran and triazophos using hybrid hybridomas. Journal of food science.2009, 74, 1-6.
41) Xiong RY*, Wu JX*, Zhou YJ, Zhou XP. Characterization and subcellular localization of an RNA silencing suppressor encoded by Rice stripe Tenuivirus. Virology, 2009, 387,29-40(IF：3. 5) (*These authors contribute equally to this paper)
42) Xiong RY, Wu JX, Zhou YJ, Zhou XP. Identification of a movement protein of rice stripe Tenuivirus. Journal of Virology，2008，82（24）:12304-12311( IF：5.4)
43) Xu YP, Wu JX*. First report of ageratum yellow vein virus infecting sonchus oleraceus. Journal of Plant Pathology. 2008, 90 (2):399. ( IF：0.974) (* corresponding author)
44) Jin RY, Gui WJ, Guo YR, Wang CM, Wu JX, Zhu GN. Comparison of Monoclonal Antibody-based ELISA for Triazophos between the Indirect and Direct Formats. Food and Agricultural Immunology, 2008, 19(1) , 49 – 60. ( IF：0.581)
45) Wu JX, Yu L, Li L , Hu JQ, Zhou JY, Zhou XP. Oral immunization with transgenic rice seeds expressing VP2 protein of infectious bursal disease virus induces protective immune responses in chickens. Plant Biotechnology Journal, 2007, 5, 570–578.( IF：5.4)
46) Xiong Q, Fan SW, Wu JX, Zhou XP. Ageratum yellow vein
47) Xiong RY, Cheng ZB, Wu JX, Zhou YJ, Zhou T., Zhou XP. First report of an outbreak of Rice stripe virus on wheat in
48) Zhou JY, Chen JG, Wang JY, Wu JX, Gong H. cDNA cloning and functional analysis of goose interleukin-2. Cytokine. 2005, 30:328-338. (IF：2.169)
49) Zhou JY, Shang SB, Gong H, Chen QX, Wu JX, Shen HG, Chen TF, Guo JQ. In vitro expression, monoclonal antibody and bioactivity for capsid protein of porcine circovirus typeⅡwithout nuclear localization signal. Journal of Biotechnology, 2005, 118:201-211. (IF：2.565)
50) Zhang DY, Zhou JY, Fang J, Hu JQ, Wu JX, Mu AX. An ELISA for antibodies to infectious bronchitis virus based on nucleocapsid protein produced in Escherichia coli. Veterinarni medicina-Czech, 2005, 50(8): 336-344.
51) Yu C, Wu JX, Zhou X P. Detection and subgrouping of cucumber mosaic virus isolates by TAS-ELISA and immunocapture RT-PCR. Journal of Virological Methods. 2005, 123: 155-161. (IF：2.0)
52) Zhou JY, Wang JY, Chen JG, Wu JX, Gong H, Teng QY, Guo JQ, Shen HG. Cloning, in vitro expression and bioactivity of duck interleukin-2. Molecular Immunology.2005, 42:589-598. (IF：3.742)
53) Zhou JY, Cheng LQ, Zheng X , Wu JX, Shuang SB, Wang JY, Chen JG. Generation of the transgenic potato expressing full-length spike protein of infectious bronchitis virus, Journal of Biotechnology, 2004，111，121-130. (IF：2.565)
54) Zhou JY，Wu JX, Cheng LQ, Zheng XJ, Gong H, Shuang SB, Zhou EM. Expression of immunogenic S1 glycoprotein of infectious bronchitis virus in transgenic potatoes, Journal of Virology, 2003, 77 (16): 9090-9093. ( IF：5.4)
55) Zhou JY, Cheng LQ, Shen XY, Ding HM, Wu JX. 2002. Cloning and Sequencing of S gene of Novel Variant of Infectious Bronchitis Virus ZJ971 Isolates in