李正和 教授
Curriculum Vitae
Prof. Zhenghe Li
866 Yuhangtang Rd
Faculty of Agriculture, Life and Environment Science Building C-949
Institute of Biotechnology
Zhejiang University,
Hangzhou 310058, China
Tel: +86-571-88982387 (O); 135-8880-1835 (C)
E-mail: lizh@zju.edu.cn
EDUCATION
2005 Ph. D.Plant Pathology, Zhejiang University, Hangzhou, Zhejiang
2000 B.S.Plant Protection, Southwest Agricultural University, Chongqing, China.
PROFESSIONAL EXPERIENCE
2011-Present Professor, State Key Laboratory of Rice Biology, Institute of Biotechnology, Zhejiang University, Hangzhou, China
2005-2011 Postdoctoral scholar, Department of Plant Pathology, University of Kentucky, USA.
2004-2005 Visiting Scholar, Institute of Resistance Research and Pathogen Diagnostics, Federal Center for Breeding Research on Cultivated Plants, Germany.
RESEARCH INTERESTS
(1) Reverse genetics systems of plant negative-strand RNA viruses.
(2) Biology of plant rhabdovirus and biotechnological applications of viral vectors.
Honors and awards
2016 “Outstanding Junior Plant Pathologist Award” of Chinese Society of Plant Pathology
2012 China National Funds for Excellent Young Scientists
2012 Zhejiang Natural Science Funds for Outstanding Young Scientists
2010 Travel award to American Society for Virology 2010, Montana University.
2008 Travel award to American Society for Virology 2008, Cornell University.
other professional activities
2017- Associate Editor, Virologica Sinica
2016- Review Editor, Frontiers in Microbiology
2012- Associate Editor, Virology Journal
2012- Member, American Society of Phytopathology
2007- Associate Member, American Society for Virology
grant support
1.National Natural Science Foundation (No. 31671996)
Title: Construction of T7 polymerase and RNA polymerase III-based reverse genetics systems for rice stripe tenuivirus
Period: 2017.1-2020.12
Amount: 720,000￥
2.National Natural Science Foundation (No. 31470255)
Title: Developing infectious cDNA clones of a plant negative-strand rhabdovirus and reverse genetics analysis
Period: 2015.1-2018.12
Amount: 860,000￥
3.The National Key Basic Research and Development Program of China (2014CB138400)
Title: Biology aspects of transmission and pathogenesis of insect-vectored plant viruses
Period: 2014.1-2018.12
Amount: 900,000￥
4.National Natural Science Foundation
Title: Saccharomyces cerevisiae as a model host to study the replication of plant viruses
Period: 2013.1-2015.12
Amount: 1,000,000￥
5.Natural Science Foundation of Zhejiang Province
Title: The role of host factor in geminivirus replication using Saccharomyces cerevisiae as a model and system biology approaches.
Period: 2012.1-2014.12
Amount: 300,000￥
6.National Natural Science Foundation
Title: Identification of cis-elements involved in the replication specificity and efficiency of betasatellite associated with monopartite geminiviruses.
Period: 2012.1-2014.12
Amount: 220,000￥
Invited Presentations at National and International Meetings
1.Li ZH*, Sun K, Zhou X. (2017) Insights into plant rhabdovirus morphogenesis and local movement. The 5th international Conference on biotic plant interaction. Xiamen, Fujian, China.
2.Sun K, Zhang XY, Zhou X, Jackson AO, Li ZH*. (2016) Glycoprotein and matrix protein requirements for budding of a plant nucleorhabdovirus. ASV Annual Meeting, Blacksburg, VA, Virginia Tech, USA
3.Li ZH*, Ma XN, Qian SS, Zhang XY, Wang Q, Sun K. (2016) Developing systems for reverse genetic analysis of plant negative-strand RNA virus. 2016 Fuzhou International Plant Pathology Symposium, Fuzhou, Fujiang , China.
4.Li ZH*, Wang Q, Ma Xiaonan, Qian SS, Sun K, Zhou XP, Jackson AO. (2015) Reverse genetic analysis of a plant negative-strand RNA virus: Insights into requirements for movement and morphogenesis of sonchus yellow net virus. ASV Annual Meeting, London, Ontario, Canada.
5.Li ZH*, Li FF, Huang CJ, Zhou XP, (2013) PTGS suppression by the tomato yellow leaf curl china betasatellite requires a host calmodulin-like protein to repress RDR6 expression. The 7th International Geminivirus Symposium, Hangzhou, China.
6.Li ZH*, Qian SS, Sun K, Ganesan U, Bragg JN, Jackson AO. (2013) Development and applications of plant negative-stranded RNA viruses reverse genetics analysis. The 3rd international Conference on biotic plant interaction. Yangling, Xian, China.
7.Li ZH, Li FF, Huang CJ, Zhou XP. (2012) Suppression of post-transcriptional gene silencing by a geminivirus-associated satellite requires a host endogenous RNA silencing suppressor rgs-CaM. ASV Annual Meeting, Madison, Wisconsin.
8.Li ZH, Nagy PD. (2010) Central role of eukaryotic translation elongation factor
9.Li ZH,Nagy PD. (2009) Genetic and biochemical evidence for the roles of eukaryotic translation elongation factor (eEF
10.Li ZH,Nagy PD. (2008) Eukaryotic translation elongation factor
11.Li ZH, Nagy PD. (2007) Cdc34p ubiquitin-conjugating enzyme is a component of the tombusvirus replicase complex. 6th International Symposium on Positive
PUBLICATIONS
1.Kovalev N, Inaba JI, Li Z, Nagy PD. The role of co-opted ESCRT proteins and lipid factors in protection of tombusviral double-stranded RNA replication intermediate against reconstituted RNAi in yeast. PLoS Pathogens. 2017, 13(7):e1006520. doi: 10.1371/journal.ppat.1006520
2.Qian S, Chen X, Sun K, Zhang Y, Li Z. 2017. Capped antigenomic RNA transcript facilitates rescue of a plant rhabdovirus. Virology Journal, 14(1):113. doi: 10.1186/s12985-017-0776-7.
3.Li F, Zhao N, Li Z, Xu X, Wang Y, Yang X, Liu SS, Wang A, Zhou X. 2017. A calmodulin-like protein suppresses RNA silencing and promotes geminivirus infection by degrading SGS3 via the autophagy pathway in Nicotiana benthamiana. PLoS Pathogens, 2017, 13(2):e1006213. doi: 10.1371/journal.ppat.1006213.
4.Wang Q, Zhang C, Wang C, Qian Y, Li Z, Hong J, Zhou X. 2017. Further characterization of Maize chlorotic mottle virus and its synergistic interaction with Sugarcane mosaic virus in maize. Scientific Reports, 7:39960.
5.Jackson AO, Li Z*. Developments in plant negative-strand RNA virus reverse genetics. Annual Review of Phytopathology, 2016, 54: 469-498.
6.Zhang T, Xu X, Huang C, Qian Y, Li Z, Zhou X*. A Novel DNA motif contributes to selective replication of a geminivirus-associated betasatellite by a helper virus-encoded replication-related protein.Journal of Virology, 2016, 90(4):2077-89.
7.Wang Q, Ma X, Qian S, Zhou X, Sun K, Chen X, Zhou X, Jackson AO, Li Z*. Rescue of a Plant Negative-Strand RNA Virus from Cloned cDNA: Insights into Enveloped Plant Virus Movement and Morphogenesis. PLoS Pathogens, 2015, 11(10): e1005223. doi: 10.1371/journal.ppat.1005223.
·This article was recommended by Faculty of 1000 Biology
8.Li F, Xu X, Huang C, Gu Z, Cao L, Hu T, Ding M, Li Z*, Zhou X*. The AC5 protein encoded by Mungbean yellow mosaic India virus is a pathogenicity determinant that suppresses RNA silencing-based antiviral defenses. New Phytologist, 2015, 208(2):555-569. doi: 10.1111/nph.13473.
9.Li F, Huang C, Li Z*, Zhou X*. Suppression of RNA Silencing by a Plant DNA Virus Satellite Requires a Host Calmodulin-like Protein to Repress RDR6 Expression. PLoS Pathogens, 2014, 10(2):e1003921. doi: 10.1371/journal.ppat.1003921.
10.Li Z, Gonzalez PA, Sasvari Z, Kinzy TG, Nagy PD*. Methylation of translation elongation factor1A by the METTL10-like See1 methyltransferase facilitates tombusvirus replication in yeast and plants. Virology, 2014, 448:43-54.
11.Li Z, Zhou X*. Small RNA biology: from fundamental studies to applications. Science China Life Science, 2013, 56:1059–1062.
12.Ganesan U, Bragg JN, Deng M, Marr S, Lee MY, Qian S, Shi M, Kappel J, Peters C, Lee Y, Goodin MM, Dietzgen RG, Li Z*, Jackson AO*. Construction of a Sonchus Yellow Net Virus Minireplicon: a Step toward Reverse Genetic Analysis of Plant Negative-Strand RNA Viruses. Journal of Virology. 2013, 87(19):10598-10611.
·This article was highlighted by Journal of Virology as Spotlight
·This article was recommended by Faculty of 1000 Biology
13.Huang CJ, Qian YJ, Li ZH, Zhou XP. Virus-induced gene silencing and its application in plant functional genomics. Sci China Life Sci. 2012, 55(2):99-108.
14.Li ZH, Nagy PD. Diverse roles of host RNA binding proteins in RNA virus replication. RNA Biology2011, 8: 305-315.
15.Li ZH, Pogany J, Panavas T, Xu K, Esposito AM, Kinzy TG, Nagy PD. Translation Elongation Factor
16.Jiang Y, Li ZH, Nagy PD. Nucleolin/Nsr1p binds to the 3' noncoding region of the tombusvirus RNA and inhibits replication. Virology2010, 396:10-20.
17.Mendu V, Chiu MX, Barajas DB, Li ZH, Nagy PD. Cpr1 cyclophilin and Ess1 parvulin prolyl isomerases interact with the tombusvirus replication protein and inhibit viral replication in yeast model host. Virology 2010, 406(2):342-51.
18.Barajas D, Li ZH, Nagy PD. The Nedd4-Type Rsp5p Ubiquitin Ligase Inhibits Tombusvirus Replication by Regulating Degradation of the p92 Replication Protein and Decreasing the Activity of the Tombusvirus Replicase. Journal of Virology2009, 83: 11751-11764.
19.Li ZH, Pogany J, Panavas T, Nagy PD et al. Translation elongation factor
20.Pogany J, Stork J, Li ZH, Nagy PD. In vitro assembly of the Tomato bushy stunt virus replicase requires the host Heat shock protein 70.Proceedings of the National Academy of Sciences the United States of America2009, 105:19956-19961.
21.Ding CJ, Qing L, Li ZH, Liu Y, Qian YJ, Zhou XP. Genetic determinants of symptoms on viral DNA satellites. Applied and Environmental Microbiology 2009, 75:5380-5389.
22.Li ZH, Barajas D, Panavas T, Nagy PD et al. Cdc34p ubiquitin-conjugating enzyme is a component of the tombusvirus replicase complex and ubiquitinates p33 replication protein. Journal of Virology 2008, 82: 6911-692.
23.Li ZH, Xie Y, Zhou XP. Tobacco curly shoot virus DNA β is not necessary for infection but may intensifies symptoms in a host-dependent manner. Phytopathology 2004, 95:902-908.
24.Li ZH, Zhou XP, Zhang X, Xie Y. Molecular characterization of tomato-infecting begomoviruses in Yunnan, China. Archives of Virology 2004, 149:1721–1732.
25.Zhou XP, Xie Y, Tao XR, Zhang ZK, Li ZH, Fauquet CM. Characterization of DNA beta associated with begomoviruses in China and evidence for co-evolution with their cognate viral DNA-A.Journal of General Virology 2003, 84: 237-247.