Jin, R. Y., W. J. Gui, et al. (2008). "Comparison of monoclonal antibody-based ELISA for triazophos between the indirect and direct formats." Food and Agricultural Immunology 19(1): 49-60.
[Abstract]: In this paper, two formats of enzyme-linked immunosorbent assays (ELISA) with the monoclonal antibody were adopted and compared to detect triazophos residue in an environmental water sample that employed a heterologous coating conjugate. The sensitivity and specificity of the two formats, indirect format (antigen-coated ELISA) and direct format (antibody-coated ELISA), were satisfied after most of the factors used to influence the analytical results of an immunoassay to triazophos were tested and optimised. Results showed that 150 and 120 were 2.54 and 0.54 ng/ml for the indirect format and 0.65 and 0. 12 ng/ml for the direct format, respectively, without obvious cross-reactivity to other organophosphorus pesticide analogues tested. The mean recoveries of water samples spiked with triazophos were 115 and 103%, for the indirect and direct format, respectively, and their mean coefficients of variation were 9.0 and 10.9%, respectively.