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Autophagy: Rice black-streaked dwarf virus P10 promotes phosphorylation of GAPDH (glyceraldehyde-3-phosphate dehydrogenase) to induce autophagy in Laodelphax striatellus


Rice viruses are prevalent in many rice-growing countries and often cause serious damages to rice production. Rice black-streaked dwarf virus (RBSDV), a member of the genus Fijivirus of family Reoviridae, is one of destructive plant viruses that causes massive loss of rice yield each year.

On July 27, 2021, the research team led by Prof. Wu Jianxiang in Institute of Biotechnology, Zhejiang University, published an article titled “Rice black-streaked dwarf virus P10 promotes phosphorylation of GAPDH (glyceraldehyde-3-phosphate dehydrogenase) to induce autophagy in Laodelphax striatellus” in Autophagy. The research illuminated the mechanism by which RBSDV induces cell autophagy in L. striatellus, and found that the autophagy pathway participates in the anti-RBSDV innate immune response in insect vector.

Professor Wu Jianxiang’s research group found that RBSDV infection can induce autophagy in L. striatellus at the early infection stage and RBSDV main capsid protein P10 is the inducer of autophagy. In the autophagy activated L. striatellus, RBSDV P10 protein and viral genome RNA transcripts were significantly decreased, suggesting that autophagy can strongly inhibit RBSDV infection and replication in L. striatellus. Then, LsGAPDH protein was found to interact with RBSDV P10 and can co-localize with P10 in L. striatellus midgut cells. Further research found that RBSDV infection or RBSDV P10 can promote phosphorylation of AMPK, resulting in GAPDH phosphorylation and relocation of phosphorylated GAPDH from the cytoplasm into the nucleus and then activate autophagy in insect cells. This study is the first report on the mechanism by which plant viruses cause autophagy in their insect vectors, which is helpful to further understand the interaction between plant viruses and their insect vectors, and to construct prevention and control strategies for viral diseases.

Prof. Wu Jianxiang, Prof. Zhou Xueping and Postdoc. Fu Shuai are the co-corresponding authors. PhD. student Wang Qi and Dr. Lu Lina are the co-first authors. This research was supported by the National Natural Science Foundation of China and the National Key Research and Development Project of China.


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Figure 1: RBSDV infection-induced autophagy in L. striatellus midguts at various days post-feeding.

Figure 2: A working model of RBSDV P10-induced autophagy in L. striatellus cells.