郭逸蓉 硕导

发布者:农药与环境毒理研究所发布时间:2017-07-19浏览次数:216




郭逸蓉, , 博士,副教授,浙江大学求是青年学者,硕士生导师。主要从事农药免疫分析化学与残留快速检测研究。先后主持或骨干参与国家自然科学基金、博士点基金、农业部行业专项等科研项目共10余项,Analytica Chimica ActaMicrochimica Acta等专业领域国际期刊发表学术论文30余篇,授权国家发明专利6项,曾获2015年全国商业科技进步一等奖、2014年浙江省科技进步二等奖各1项。


联系方式:0571-88982517, yirongguo@zju.edu.cn

  

20056月本科毕业于华中农业大学植物保护系;20106月获浙江大学农药学专业博士学位;20111-20132月在比利时根特大学药学院食品分析实验室从事博士后研究工作,受欧盟第七框架计划MYCOHUNT项目资助;20133月入职浙江大学农药与环境毒理研究所。目前研究方向为农产品质量安全监控,主要从事小分子化合物免疫化学、分子印迹技术等食品安全快速检测技术研究,研究对象包括农药、兽药和生物毒素等多类环境及食品污染物。

  

主持项目:

l国家青年科学基金项目:农药多残留上转发光纳米晶技术免疫层析快速检测(31401768

l高等学校博士学科点专项科研基金(新教师类):草甘膦和百草枯的分子印迹金标层析法仿生快速检测技术20130101120117

l浙江省公益技术应用研究项目:茶叶中农药多残留速测免疫芯片技术研发(2016C32004)

l中央高校基本科研业务费专项:农药多残留免疫芯片技术研究(2014QNA6022

l浙江省教育厅科研项目:含氰基拟除虫菊酯类农药的免疫快速检测技术(Y201329970

l浙江大学现代光学仪器国家重点实验室开放课题: 基于双环谐振腔光学传感器的农药残留免疫速测技术

  

主要参与项目:

l国家重点研发计划项目:“化肥农药减施增效技术应用及评估研究”子课题(2016YFD0201302

l农业部公益性行业科研专项:“农产品安全快速监测技术和产品研发及其示范应用”子课题(201203094-3

l欧盟第七框架计划(FP7/2007-2013MYCOHUNT项目:小麦中真菌毒素快速检测生物传感器研制(243633

l国家863项目:分子印迹在农药残留检测中的应用研究(2008AA10Z422

l国家自然科学基金:农药多簇人工抗原的免疫特异性宽谱应答及多残留免疫金技术(30671391

l博士学科点专项科研基金:有机磷杀虫剂半抗原分子设计与免疫活性的构效关系(20050335116

l国家自然科学基金:农药残留免疫化学分析的半抗原分子设计及其免疫识别机制研究(30370944

所获科研奖励:

农产食品中有害物质的免疫与分子快速检测技术研究及应用(J-2-0472014年度浙江省科学技术进步二等奖(排名:3/9)和2015年度全国商业科技进步一等奖(排名:3/12

发表学术论文(*为通讯作者)

40)邹茹冰; 柳颖; 王双节; 张亚; 郭逸蓉*;朱国念,化学发光酶联免疫分析法同时检测三种有机磷农药残留. 农药学学报2017, 19, (1), 1-9.

39) 胡静; 郭逸蓉; 梁晓; 刘贤金; 朱国念; 刘凤权; 王鸣华; 王利民; 华修德; 张存政, 菊酯类农药广谱型免疫层析试纸条的研究及应用. 分析化学2016, 44, (12), 1900-1906.

38) Sun, Q.; Wang, Y.; Tian, C.; Gui, W.; Guo, Y. *; Zhu, G., Residue analysis of benzobicyclon in soil and sediment samples by ultra high-performance liquid chromatography-tandem mass spectrometry, Journal of AOAC International, 2016, 99, (6), 1628-1635.

37) Lan, M.; Guo, Y.*; Zhao, Y.; Liu, Y.; Gui, W.; Zhu, G., Multi-residue detection of pesticides using a sensitive immunochip assay based on nanogold enhancement. Analytica Chimica Acta2016, 938, 146-155.

36) Dong, M.; Si, W.; Wang, W.; Bai, B.; Nie, D.; Song, W.; Zhao, Z.; Guo, Y.*; Han, Z., Determination of type A trichothecenes in coix seed by magnetic solid-phase extraction based on magnetic multi-walled carbon nanotubes coupled with ultra-high performance liquid chromatography-tandem mass spectrometry. Analytical and Bioanalytical Chemistry2016, 408, (24), 6823-6831.

35) 刘蕊; 项丹丹; 刘鹏琰; 梁晓; 郭逸蓉; 朱国念, 抗对硫磷单链抗体在昆虫细胞中的表达及活性鉴定. 农药学学报2016, 18, (2), 177-184.

34)赵颖; 杨斌; 柳颖; 方一画; 司芳芳; 郭逸蓉*; 程敬丽; 朱国念, 啶虫脒金标免疫速测试纸条研制及其在茶叶中的应用, 农药学学报, 2016, 18, (3), 337-343.

33)Liu, Y.; Liu, R.; Boroduleva, A.; Eremin, S.; Guo, Y.*; Zhu, G., A highly specific and sensitive fluorescence polarization immunoassay for the rapid detection of triazophos residue in agricultural products. Analytical Methods2016, 8, (36), 6636-6644.

32) Liu, R.; Liang, X.; Xiang, D.; Guo, Y.*; Liu, Y.; Zhu, G., Expression and functional properties of an anti-triazophos high-affinity single-chain variable fragment antibody with specific lambda light chain. International Journal of Molecular Sciences2016, 17, (6), 823.

31) Wang, Q.; Wei, P.; Cao, M.; Liu, Y.; Wang, M.;Guo, Y.*; Zhu, G., Residual behavior and risk assessment of the mixed formulation of benzene kresoxim-methyl and fluazinam in cucumber field application. Environmental Monitoring and Assessment2016, 188, (6), 1-10.

30) Sanders, M.; McPartlin, D.; Moran, K.; Guo, Y.; Eeckhout, M.; O Kennedy, R.; De Saeger, S.; Maragos, C., Comparison of enzyme-linked immunosorbent assay, surface plasmon resonance and biolayer interferometry for screening of deoxynivalenol in wheat and wheat dust. Toxins2016, 8, (4), 103.

29)  Taheri, N.; Lan, M.; Wei, P.; Liu, R.; Gui, W.; Guo, Y.*; Zhu, G., Chemiluminescent enzyme immunoassay for rapid detection of three α-cyano pyrethroid residues in agricultural products. Food Analytical Methods 2016, 9, (10), 2896-2905.

28)  Liu, R.; Liu, Y.; Lan, M.; Taheri, N.; Cheng, J.; Guo, Y. *; Zhu, G., Evaluation of a water-soluble adjuvant for the development of monoclonal antibodies against small-molecule compounds. Journal of Zhejiang University Science B2016, 17, (4), 282-293.

27)  Liu, Y.; Mo, R.; Tang, F.; Fu, Y.; Guo, Y.*, Influence of different formulations on chlorpyrifos behavior and risk assessment in bamboo forest of China. Environmental Science and Pollution Research2015, 22, 20245–20254.

26)  Sanders, M.; Guo, Y.; Iyer, A.; Ruiz García, Y.; Galvita, A.; Heyerick, A.; Deforce, D.; Risseeuw, M. D. P.; Van Calenbergh, S.; Bracke, M.; Eremin, S.; Madder, A.; De Saeger, S., An immunogen synthesis strategy for the development of specific anti-deoxynivalenol monoclonal antibodies. Food Additives & Contaminants: Part A2014, 31, (10), 1751-1759.

25)  Guo, Y. *; Sanders, M.; Galvita, A.; Heyerick, A.; Deforce, D.; Bracke, M.; Eremin, S.; De Saeger, S., Heterologous screening of hybridomas for the development of broad-specific monoclonal antibodies against deoxynivalenol and its analogues. World Mycotoxin Journal 2014, 7, (3), 257-265.

24)  Liu, Y.; Guo, Y.; Zhu, G.; Tang, F.; Enzyme-linked immunosorbent assay for the determination of five organophosphorus pesticides in camellia oil. Journal of Food Protection 2014, 77, (7), 1178-1183.

23)  Guo, Y.; Tian, J.; Liang, C.; Zhu, G.; Gui, W., Multiplex bead-array competitive immunoassay for simultaneous detection of three pesticides in vegetables. Microchimica Acta2013, 180, (5-6), 387-395.

22)  Guo, Y.; Liang, X.; Wang, Y.; Liu, Y.; Zhu, G.; Gui, W., Cyclodextrin-based molecularly imprinted polymers for the efficient recognition of pyrethroids in aqueous media. Journal of Applied Polymer Science2013, 128, (6), 4014-4022.

21)  Beloglazova N.V., De Boevre M., Goryacheva I.Yu., Werbrouck S., Guo Y., De Saeger S., Immunochemical approach for zearalenone-4-glucoside determination. Talanta, 2013, 106, 422-430.

20)  Jin, M.; Zhu, G.; Jin, R.; Liu, S.; Shao, H.; Jin, F.; Guo, Y.; Wang, J. *, A sensitive chemiluminescent enzyme immunoassay for carbofuran residue in vegetable, fruit and environmental samples. Food and Agricultural Immunology2013, 24, (3), 345-356.

19)  Liu, Y. H.; Xie, R.; Guo, Y. R.; Zhu, G. N.; Tang, F. B., Comparison of homologous and heterologous formats in nanocolloidal gold-based immunoassays for parathion residue determination. Journal of Environmental Science and Health, Part B: Pesticides, Food Contaminants, and Agricultural Wastes 2012, 47, (5), 475-483.

18)  Liu, Y. H.; Chen, J.; Guo, Y. R.; Wang, C. M.; Liang, X.; Zhu, G. N., A sensitive monoclonal antibody-based enzyme-linked immunosorbent assay for chlorpyrifos residue determination in Chinese agricultural samples. Journal of Environmental Science and Health, Part B: Pesticides, Food Contaminants, and Agricultural Wastes2011, 46, (4), 313-320.

17)  Liu, Y. H.; Guo, Y. R.; Wang, C. M.; Gui, W. J.; Zhu, G. N., Homology modeling of anti-parathion antibody and its interaction with organophosphorous pesticides and analogues. Journal of Environmental Science and Health, Part B: Pesticides, Food Contaminants, and Agricultural Wastes2010, 45, (8), 819 – 827.

16)  Gui, W.; Liang, C.; Guo, Y.; Zhu, G., An improved rapid on-Site immunoassay for triazophos in environmental samples. Analytical Letters2010, 43, (3), 487-498.

15)  Liu, Y. H.; Chen, J.; Wang, C. M.; Guo, Y. R.; Liang, X.; Zhu, G. N., Specific antibody for pesticide residue determination produced by antibody-pesticide complex. Journal of Environmental Science and Health, Part B: Pesticides, Food Contaminants, and Agricultural Wastes2010, 45, (6), 540-546.

14)  Liu, Y. H.; Wang, C. M.; Guo, Y. R.; Liang, X.; Gui, W.; Zhu, G. N., Monoclonal antibody produced by heterologous indirect enzyme-linked immunosorbent assay and its application for parathion residue determination in agricultural and environmental samples. Journal of Environmental Science and Health, Part B: Pesticides, Food Contaminants, and Agricultural Wastes2010, 45, (6), 547-556.

13)  Wang, C.; Li, X.; Liu, Y.; Guo, Y.; Xie, R.; Gui, W.; Zhu, G., Development of a Mab-based heterologous immunoassay for the broad-selective determination of organophosphorus pesticides. Journal of Agricultural and Food Chemistry2010, 58, (9), 5658-5663.12.

12)  Guo, Y.; Liu, S.; Gui, W.; Zhu, G., Gold immunochromatographic assay for simultaneous detection of carbofuran and triazophos in water samples. Analytical Biochemistry2009, 389, (1), 32-39.

11)  Jin, R.; Guo, Y.; Wang, C.; Wu, J.; Zhu, G., Development of a bispecific monoclonal antibody to pesticide carbofuran and triazophos using hybrid hybridomas. Journal of Food Science2009, 74, (1), T1-T6.

10)  Wang, C.; Liu, Y.; Guo, Y.; Liang, C.; Li, X.; Zhu, G., Development of a McAb-based immunoassay for parathion and influence of the competitor structure. Food Chemistry2009, 115, (1), 365-370.

09)  Gui, W.; Jin, M.; Sun, L.; Guo, Y.; Zhu, G., Residues determination of carbofuran in vegetables based on sensitive time-resolved fluorescence immunoassay. Food and Agricultural Immunology2009, 20, (1), 49-56.

08)  Gui, W. J.; Wang, S. T.; Guo, Y. R.; Zhu, G. N., Development of a one-step strip for the detection of triazophos residues in environmental samples. Analytical Biochemistry2008, 377, (2), 202-208.

07)  Jin, R. Y.; Gui, W. J.; Guo, Y. R.; Wang, C. M.; Wu, J. X.; Zhu, G. N., Comparison of monoclonal antibody-based ELISA for triazophos between the indirect and direct formats. Food and Agricultural Immunology2008, 19, (1), 49-60.

06)  Zhu, G.; Jin, M.; Gui, W.; Guo, Y.; Jin, R.; Wang, C.; Liang, C.; Liu, Y.; Wang, S., Development of a direct competitive enzyme-linked immunoassay for carbofuran in vegetables. Food Chemistry2008, 107, (4), 1737-1742.

05)  王姝婷,桂文君,郭逸蓉,朱国念. 氯霉素速测金标试纸条及其保护剂配方研究. 中国农业科学,200841, (12), 4372-4380.

04)  Wang, S. T.; Gui, W. J.; Guo, Y. R.; Zhu, G. N., Preparation of a multi-hapten antigen and broad specificity polyclonal antibodies for a multiple pesticide immunoassay. Analytica Chimica Acta2007, 587, (2), 287-292.

03)  Liu, Y. H.; Jin, M. J.; Gui, W. J.; Cheng, J. L.; Guo, Y. R.; Zhu, G. N., Hapten design and indirect competitive immunoassay for parathion determination: Correlation with molecular modeling and principal component analysis. Analytica Chimica Acta2007, 591, (2), 173-182.

02)  杨挺,王姝婷,郭逸蓉,王美英,朱国念. 动物源食品中氯霉素残留速测金标试纸条的研制. 中国农学通报,200723, (11), 156-161.

01)  郭逸蓉,桂文君,王姝婷,朱国念. 氯霉素ELISA检测试剂盒研制及其性能测试. 中国食品学报,2007, 7, (6), 118-123.

  

授权发明专利:

06) 桂文君, 谢蓉, 郭逸蓉, 刘菲菲, 朱国念. 莠去津分子印迹金标试纸条及其用途. 授权日2014.11.26, 专利号:ZL 201210240272.X

05) 桂文君, 郭逸蓉, 梁晓, 王阳阳, 刘毅华, 朱国念. 三氟氯氰菊酯分子印迹聚合物的制备方法及其用途. 授权日2012.4.25, 专利号:ZL 201010268194.5

04) 桂文君, 朱国念, 王姝婷, 郭逸蓉, 金仁耀. 多簇抗原及制备方法、宽谱特异性多克隆抗体及其用途. 授权日2011.6.22, 专利号:ZL 200610053008.X

03) 刘毅华, 赵金浩, 王春梅, 郭逸蓉, 桂文君, 程敬丽, 朱国念. 一种乙基对硫磷竞争半抗原和竞争原及其用途. 授权日2012.4.25, 专利号:ZL 200810062154.8

02) 郭逸蓉, 王美英, 王姝婷,杨挺,朱国念. 复合保护剂及其用途. 授权日2011.5.4,专利号:ZL 200710071012.3

01) 朱国念, 刘毅华, 桂文君, 程敬丽, 金茂俊, 郭逸蓉. 乙基对硫磷人工半抗原、人工抗原、抗体制备法及其用途.授权日2008.11.19, 专利号:ZL 200610052531.0

  

  

学术报告:

l农药多残留免疫快速筛检技术研究,大会报告,中国仪器仪表学会分析仪器分会快速检测技术及仪器专业委员会第一届学术研讨会20141016-17日,浙江嘉兴

lOral presentation, Heterologous screening of hybridomas for the development of monoclonal antibodies against deoxynivalenol and its analogues,XI International Conference on AgriFood Antibodies (ICAFA), September 3-5th 2012, Vienna, Austria

lPoster presentation, Development of monoclonal antibodies against deoxynivalenol using different antigens,4th Scientific Afternoon in the Faculty of Pharmaceutical Sciences, Ghent University, 16th May, 2012

lCyclodextrin-based molecularly imprinted polymers for selective recognition of lambda-cyhalothrin in aqueous media,MIP2010: The 6th International Conference on Molecular Imprinting, August 9-12, 2010,New Orleans, LA, U.S.A. (abstract accepted for oral presentation)