桂文君 副所长，博导

姓名：桂文君

职称：教授

职务：副所长

地址：杭州市余杭塘路866号

邮编： 310058

电话： 0571-88982517

Email： guiwj @zju.edu.cn

招生专业

植物保护

研究方向

农药环境毒理学

教育背景

工作简历

个人简介

桂文君，男，1973年生，博士，硕导，副教授，现任浙江大学农药与环境毒理研究所副所长兼党支部书记。多年来一直从事农药免疫化学、农药残留与食品安全、农药环境行为、农药水生生态毒理等方面的研究。近年来，主持国家自然科学基金面上项目1项，浙江省自然科学基金项目1项，浙江省创新团队项目子课题1项，教育部博士点新教师基金项目1项，重大横向课题4项，其它各类课题50余项。在国内外期刊发表研究论文30余篇，授权国家发明专利8项、著作1本，获浙江省科技二等奖1次，国家质检总局“科技兴检奖”一等奖1次。

主持主要项目研究

1.戊唑醇对斑马鱼HPG轴内分泌干扰作用的分子机制，国家自然科学基金面上项目，2016.1－2019.12，60万元；

2.柑橘中农药残留试验田间标准操作规程验证项目，农业部，2013.3－2013.12，6.0万元；

3.制定杀虫双在甘蔗中的残留限量：开展杀虫双在甘蔗上的残留试验，农业部，2013.1－2013.12，15万元；

4.制定嘧啶肟草醚在水稻中的残留限量，农业部，2013.1－2013.12，7万元；

5.制定砜嘧磺隆在农产品中的最大残留限量以及开展杀虫单在番茄上的残留研究及限量，农业部，2012.1－2012.12，22万元；

6.基于生物功能分析的乙酰胆碱受体多目标竞争分析方法及其在食品安全中的应用，博士点新教师基金，2012.1－2014.12，4.0万元；

7.农产品中农兽药的生物化学法快速检测技术研究，浙江省创新团队子课题，2011. 4－2014. 3，28.0万元；

8.基于分子印迹的农药新型竞争性金标试纸条快速检测技术，浙江省自然科学基金面上项目，2011.1－2012.12，6.0万元；

9.30%烯草酮乳油等农药环境毒理试验研究，企业委托，2010.7－2011.7，90万元；

10.35%草甘膦水剂等农药环境毒理试验研究，企业委托，2010.7－2011.7，54万元；

11.10%磺草酮·莠去津悬浮剂等农药登记环境毒理试验研究，企业委托(重大横向)，2010.5－2011.5，126万元；

12.农药登记环境毒理、行为试验研究，企业委托(重大横向)，2008.6－2009.7，120万元；

13.95%醚菌酯原药等对非靶生物毒性及环境行为研究，企业委托，2010.7－2011.7，79.5万元；

14.2种农药制剂的配方优化及对非靶生物毒性研究，企业委托，2010.3－2010.12，68.6万元；

15.15%乙氧磺隆水分散性粒剂研究，企业委托，2009.5－2009.12，53万元；

16.农药制剂配方及加工工艺研究，企业委托(重大横向)，2009.4－2010.12，130万元；

17.分子印迹在农药残留检测中的应用研究，重大横向(重大横向)，2008.12－2010.6，120万元；

发表论文

第一作者或通讯作者

[1]Gui, Wenjun; Tian, Chunxia; Sun, Qianqian; Li, Shuying; Zhang, Wei; Tang, Jun; Zhu, Guonian. Simultaneous determination of organotin pesticides by HPLC-ICP-MS and their sorption, desorption, and transformation in freshwater sediments. WATER RESEARCH, 2016, 95: 185-194.

[2]Yang, Mei; Hu, Jingjin; Li, Shuying; Ma, Youning; Gui, Wenjun*; Zhu, Guonian. Thyroid endocrine disruption of acetochlor on zebrafish (Danio rerio) larvae. JOURNAL OF APPLIED TOXICOLOGY, 2016,36(6): 844-852.

[3]Wang, Yihu; Sun, Qianqian; Tian, Chunxia; Gui, Wenjun*; Zhu, Guonian. Degradation properties and identification of metabolites of 6-Cl-PMNI in soil and water. CHEMOSPHERE, 2016, 147: 287-296

[4]Li, Meng; Cao, Chuyan; Li, Shuying; Gui, Wenjun*; Zhu, Guonian. Thyroid endocrine disruption of azocyclotin to Xenopus laevis during metamorphosis. ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY, 2016, 43: 61-67

[5]Liang, Xiao; Yu, Liang; Gui, Wenjun*; Zhu, Guonian. Exposure to difenoconazole causes changes of thyroid hormone and gene expression levels in zebrafish larvae. ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY, 2015, 40(3): 983-987.

[6]Li Shuying; Wang Yihu; Sun Qianqian;Gui Wenjun*; Zhu Guonian. Residue determination of plant activator benzo-1,2,3-thiadiazole-7-carboxylic acid 2-benzoyloxyethyl ester in water, soil, and vegetable by gas chromatography with tandem mass spectrometry. JOURNAL OF SEPARATION SCIENCE, 2015, 38(11): 1900-1906

[7]Meijing Lan, Shuying Li, Zhenzhen Xu, Chunxia Tian, Mei Yang, Wenjun Gui*, Guonian Zhu. Simultaneous determination of saflufenacil and its two metabolites in soil samples by ultra-performance liquid chromatography with tandem mass spectrometry. Journal of Separation. Science.2014, 37:658–664.

[8]Gui Wen-jun, Tian Jie, Tian Chun-xia, Li Shu-ying, Ma You-ning, Zhu Guo-nian. Ultra Performance Liquid Chromatography-tandem Mass Spectrometry for the Determination of Amicarthiazol Residues in Soil and Water Samples. Journal of Chromatography B,2014, 972:102-110.

[9]Yirong Guo, Jie Tian  Chizhou Liang，Guonian Zhu, Wenjun Gui*. Multiplex bead-array competitive immunoassay for simultaneous detection of three pesticides in vegetables. Microchimica Acta, 2013, 180:387-395.

[10]Yirong Guo, Xiao Liang, Yangyang Wang, Yihua Liu, Guonian Zhu, Wenjun Gui*. Cyclodextrin-based molecularly imprinted polymers for the efficient recognition of pyrethroids in aqueous media. Journal of Applied Polymer Science, 2013, 128(6): 4014-4022.

[11]Gui Wenjun, Dong Qiaoxiang, Zhou Shengli, et al.WATERBORNE EXPOSURE TO CLODINAFOP-PROPARGYL DISRUPTS THE POSTERIOR AND VENTRAL DEVELOPMENT OF ZEBRAFISH EMBRYOS. Environmental Toxicology and Chemistry, 2011, 30(7): 1576-1581. 

[12]Gui Wenjun, Xu Yong, Shou Linfei, et al. Liquid chromatography-tandem mass spectrometry for the determination of chrysoidine in yellow-fin tuna. Food Chemistry, 2010, 122(4): 1230-1234.  

[13]Gui Wenjun, Liang Chizhou, Guo Yirong, et al.AN IMPROVED RAPID ON-SITE IMMUNOASSAY FOR TRIAZOPHOS IN ENVIRONMENTAL SAMPLES.Analytical Letters, 2010, 43(3): 487-498 

[14]Gui Wen-Jun, Liu Yi-Hua, Wang Chun-Mei, et al.Development of a direct competitive enzyme-linked immunosorbent assay for parathion residue in food samples. Analytical Biochemistry, 2009, 393(1): 88-94

[15]Gui Wenjun, Jin Maojun, Sun Lifeng, et al. Residues determination of carbofuran in vegetables based on sensitive time-resolved fluorescence immunoassay. Food and Agricultural Immunology, 2009,20(1): 49-56

[16]Gui Wen-Jun, Wang Shu-Ting, Guo Yi-Rong, et al.Development of a one-step strip for the detection of triazophos residues in environmental samples.Analytical Biochemistry, 2008, 377(2): 202-208

[17]Gui Wen Jun, Jin Ren Yao, Chen Ze Li, et al. Hapten synthesis for enzyme-linked immunoass- ay of the insecticide triazophos. Analytical Biochemistry, 2006, 357(1):9-14 

共同作者

[18]Liu, Hongcu; Chu, Tianyi; Chen, Lili; Gui, Wenjun; Zhu, Guonian.In vivo cardiovascular toxicity induced by acetochlor in zebrafish larvae. CHEMOSPHERE, 2017, 181: 600-608.

[19]Li, Shuying; Li, Meng; Gui, Wenjun; Wang, Qiangwei; Zhu, Guonian. Disrupting effects of azocyclotin to the hypothalamo-pituitary-gonadal axis and reproduction of Xenopus laevis. AQUATIC TOXICOLOGY, 2016, 185: 121-128.

[20]Sun, Qianqian; Wang, Yehu; Tian, Chunxia; Gui, Wenjun; Guo, Yirong; Zhu, Guonian. Residue Analysis of Benzobicyclon in Soil and Sediment Samples by Ultra High-Performance Liquid Chromatography-Tandem Mass Spectrometry. JOURNAL OF AOAC INTERNATIONAL, 2016, 99(6) : 1628-1635

[21]Taheri, Niusha; Lan, Meijing; Wei, Peng; Liu, Rui; Gui, Wenjun; Guo, Yirong; Zhu, Guonian.Chemiluminescent Enzyme Immunoassay for Rapid Detection of Three alpha-Cyano Pyrethroid Residues in Agricultural Products. FOOD ANALYTICAL METHODS, 2016, 9(10): 2896-2905.

[22]Lan, Meijing; Guo, Yirong; Zhao, Ying; Liu, Yihua;Gui, Wenjun; Zhu, Guonian. Multi-residue detection of pesticides using a sensitive immunochip assay based on nanogold enhancement. ANALYTICA CHIMICA ACTA, 2016, 938: 146-155   

[23]Li, Shuying; Li, Meng; Wang, Qiangwei; Gui, Wenjun; Zhu, Guonian. Exposure to butachlor causes thyroid endocrine disruption and promotion of metamorphosis in Xenopus laevis. CHEMOSPHERE, 2016, 152: 158-165

[24]Yang, Mei; Ma, Youning; Gui, Wenjun; Ren, Yiping; Zhu, Guonian; Liu, Yihua. Determination of 26 endocrine disrupting chemicals in fish and water using modified QuEChERS combined with solid-phase extraction and UHPLC-MS/MS. ANALYTICAL METHODS, 2015, 7 (19): 8418-8431.

[25]Yu, Liang; Chen, Mengli; Liu, Yihua; Gui, Wenjun; Zhu, Guonian. Thyroid endocrine disruption in zebrafish larvae following exposure to hexaconazole and tebuconazole, AQUATIC TOXICOLOGY, 2013, 138:35-42

[26]Liang, Chizhou; Zou, Mingqiang; Guo, Lianghong; Gui, Wenjun; Zhu, Guonian. Development of a bead-based immunoassay for detection of triazophos and application validation. FOOD AND AGRICULTURAL IMMUNOLOGY, 2013, 24(1): 9-20

[27]Liang, Xiao; Xie, Rong; Wang, Chunmei; Gui, Wenjun; Zhu, Guonian. Development of a broad-selective immunoassay for multi-residue determination of type II pyrethroids in West Lake water. FOOD AND AGRICULTURAL IMMUNOLOGY, 2013, 24(1): 59-78

[28]Jin, Maojun; Shao, Hua; Jin, Fen; Gui, Wenjun; Zhu, Guonian. Enhanced Competitive Chemiluminescent Enzyme Immunoassay for the Trace Detection of Insecticide Triazophos. JOURNAL OF FOOD SCIENCE, 2012, 77(5):T99-T104.

[29]Chang, Juhua; Wang, Minghua; Gui, Wenjun; Zhu, Guonian. Changes in Thyroid Hormone Levels during Zebrafish Development. ZOOLOGICAL SCIENCE, 2012, 29(3): 181-184

[30]Chang, Juhua; Gui, Wenjun; Wang, Minghua; Zhu, Guonian. Effects of butachlor on estrogen receptor, vitellogenin and P450 aromatase gene expression in the early life stage of zebrafish. JOURNAL OF ENVIRONMENTAL SCIENCE AND HEALTH A. 2012, 47(11): 1672-1677.

[31]Liu, Yihua; Xu, Zhenzhen; Wu, Xiaoguang; Gui, Wenjun; Zhu, Guonian. Adsorption and desorption behavior of herbicide diuron on various Chinese cultivated soils. JOURNAL OF HAZARDOUS MATERIALS, 2010, 178(1-3):462-468.

[32]Wang, Chunmei; Li, Xianbin; Liu, Yihua; Gui, Wenjun; Zhu, Guonian. Development of a Mab-Based Heterologous Immunoassay for the Broad-Selective Determination of Organophosphorus Pesticides. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2010, 58(9):5658-5663.

[33]Liu, Shaoying; Zheng, Zuntao; Wei, Fanglin; Gui, Wenjun; Zhu, Guonian. Simultaneous Determination of Seven Neonicotinoid Pesticide Residues in Food by Ultraperformance Liquid Chromatography Tandem Mass Spectrometry. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2010, 58(6):3271-3278

[34]Shi, Chenhui; Gui, Wenjun; Chen, Jie; Zhu, Guonian. Determination of Oxadiargyl Residues in Environmental Samples and Rice Samples. BULLETIN OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY, 2010, 84(2):236-239

[35]Liu, Yi H.; Wang, Chun M.; Guo, Yi R.; Gui, Wen J; Zhu, Guo N. Monoclonal antibody produced by heterologous indirect enzyme-linked immunosorbent assay and its application for parathion residue determination in agricultural and environmental samples. JOURNAL OF ENVIRONMENTAL SCIENCE AND HEALTH  B, 2010, 45(6):547-556

[36]Liu, Yi H.; Guo, Yi R.; Wang, Chun M.; Gui, Wen J; Zhu, Guo N. Homology modeling of anti-parathion antibody and its interaction with organophosphorous pesticides and analogues. JOURNAL OF ENVIRONMENTAL SCIENCE AND HEALTH B, 2010, 45(8):819-827

[37]Liu, Yihua; Wang, Chunmei; Gui, Wenjun; Zhu, Guonian. Development of a sensitive competitive indirect ELISA for parathion residue in agricultural and environmental samples. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY, 2009, 72(6)1673-1679

[38]Guo, Yi-Rong; Liu, Shao-Ying; Gui, Wen-Jun; Zhu, Guonian. Gold immunochromatogra- phic assay for simultaneous detection of carbofuran and triazophos in water samples. ANALYTICAL BIOCHEMISTRY, 2009, 389(1):32-39

编著

朱国念，吴慧明，桂文君.农药残留快速检测技术. 北京：化学工业出版社，2008

授权发明专利

1.桂文君，郭逸蓉，梁晓，王阳阳，刘毅华，朱国念. 三氟氯氰菊酯分子印迹聚合物的制备方法及其用途. 专利号：ZL 201010268194.5 （授权日期：2012.4.25）

2.桂文君，朱国念，王姝婷，郭逸蓉，金仁耀. 多簇抗原、宽谱特异性多克隆抗体的制备方法及其用途，专利号：ZL 200610053008.X（授权日期：2011.6.22）

3.桂文君，程敬丽，朱国念. 三唑磷人工半抗原、抗原、特异性抗体及其用途，专利号：ZL 200510048968.2. （授权日期：2007.10.3）

4.朱国念，桂文君，程敬丽. 三唑磷人工半抗原、抗原、抗体的制备方法，专利号： ZL 200510048970.X. （授权日期：2007.12.12）

5.刘毅华，朱国念，桂文君，程敬丽，金茂俊，郭逸蓉. 乙基对硫磷人工半抗原、人工抗原、抗体制备法及其用途，专利号： ZL 200610052531.0.  （授权日期：2008.11.19）

6.朱国念，金仁耀，程敬丽，桂文君，陈则利，金茂俊，王春梅，氰戊菊酯人工抗原、抗体及其用途，专利号： ZL 200510061971.8. （授权日期：2007. 8.15）

7.程敬丽，朱国念，朱烈，桂文君，金仁耀，金茂俊. 氰戊菊酯半抗原化合物、合成方法及其用途，专利号：ZL 200510061972.2. （授权日期：2007.10.3）

科研奖励

1.桂文君(3/10). 农产食品中有毒有害物质快速检测关键技术研究及应用. 国家质检总局, 科技兴检奖，一等奖，2014；

2.桂文君(5/9). 蔬菜质量安全监控与标准化技术研究与应用. 浙江省科技二等奖，2010年；

3.桂文君(6/12). 蔬菜安全生产和质量监控关键技术的集成及示范. 浙江省农业厅技术进步二等奖，2009年；