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方琦  

副教授,硕士生导师,浙江大学求是青年学者

杭州市西湖区余杭塘路866

浙江大学紫金港校区农生环组团C1149

邮编:310058

电话:0571-88982696

E-mail: fangqi@zju.edu.cn





简历

受教育经历:

2006/09-2010/09,浙江大学,农业昆虫与害虫防治专业,博士;

2004/09-2006/06,浙江大学,农业昆虫与害虫防治专业,硕士;

2000/09-2004/06,浙江大学,植物保护系,学士。

研究工作经历:

2018/01-至今,   浙江大学,昆虫科学研究所,副教授;

2013/12-2017/12,浙江大学,昆虫科学研究所,助理研究员;

2013/01-2013/11,浙江大学,昆虫科学研究所,助理研究员,博士后;

2012/01-2013/01,杜伦大学,生物医学与生物科学学院,访问学者;

2010/11-2012/01,浙江大学,昆虫科学研究所,博士后。


研究领域

主要从事植物害虫生物防治和昆虫免疫学方向的研究。近年来的研究重点为寄生蜂雌蜂所携活性因子的分离、鉴定与功能研究,鳞翅目农业害虫的天然免疫反应及其调控机理,以及寄生蜂与其寄主害虫相互作用的机理等。目前的研究工作主要包括以下几个方面:1)金小蜂科寄生蜂毒液蛋白组分的分离、鉴定与功能解析;2)鳞翅目农业害虫细胞与体液免疫反应过程及其调控机理;3)寄生蜂毒液蛋白组分与其寄主天然免疫反应靶标间的相互作用;4)节肢动物功能组学及其在植物害虫生物防治中的前期应用基础。


目前承担的项目

项目名称

项目性质及来源

研究期限

蝶蛹金小蜂毒液pacifastin类蛋白酶抑制因子调控寄主免疫反应机理(主持)

浙江省自然科学基金面上项目(LY18C140001

201801月至202012

蝶蛹金小蜂毒液源Kazal型蛋白酶抑制因子功能研究及其应用初探(主持)

中央高校基本科研业务费项目2017FZA6013

201701月至201812

农作物重要病毒病昆虫传播与致害的生物学基础:课题4-介体昆虫—病毒—寄主植物三者互作机制(研究骨干)

973计划课题(2014CB138404

201401月至201808

蝶蛹金小蜂毒液富半胱氨酸结构域蛋白的功能及其作用机理(参加)

国家自然科学基金面上项目(31472038

201501月至201812

农业科研人才支撑计划(研究骨干)

农业部

2016RA4008

201601月至20201231

甲脒类杀虫剂的分子靶标研究(参加)

国家自然科学基金面上项目(31572039

201601月至201912


教学工作

参与讲授的研究生课程有《植物病虫生理学(昆虫)》全英文课程和《昆虫生化与分子生物学》。参与讲授的本科生课程为《植物保护学II(园艺)》

已招收指导硕士研究生生2人,指导本科生毕业论文2人。协助课题组长叶恭银教授指导博士和硕士研究名。


国际交流合作

作为中方主要研究骨干参与中英联合项目(BBSRC-China Partnering Award, Grant no. BB/G530409/1, 01/04/2009-31/03/2013

多次参加国际学术会议并做口头报告。

  1. Fang Qi (分会报告). Identification of encapsulation-related genes from hemocytes in Pieris rapae pupae, whose expression were suppressed by venom from Pteromalus puparum (Hymenoptera: Pteromalidae). First International Symposium on Insect Physiology, Biochemistry and Molecular Biology. China, Sept. 19-22, 2007.

  2. Fang Qi (分会报告). Expression of early immune-response genes in lepidopteran host are suppressed by venom from an endoparasitoid, Pteromalus puparum. The 6th Asia-Pacific Congress of Entomology. China, Oct. 18-22, 2009.

  3. Fang Qi (分会报告).Pteromalus puparum venom inhibits host cellular immune responses via decreasing the host scavenger receptor gene expression. Third International Symposium on Insect Physiology, Biochemistry and Molecular Biology. China, Jul. 02-05, 2011.

  4. Fang Qi (分会报告). Functional studies on pacifastin proteinase inhibitor genes, NvPP-1 and NvPP-2, of Nasonia vitripennis. Forth International Symposium on Insect Physiology, Biochemistry and Molecular Biology. China, Jun. 15-19, 2013.

  5. Fang Qi (分会报告). Effects of the endoparasitoid Cotesia chilonis parasitism, venom, and calyx fluid on immunity of its host Chilo suppressalis larvae. 2016 XXV International Congress of Entomology. Orlando, the United States, Sept. 25-30, 2016.


发表论著

已发表学术论文50余篇(含SCI期刊及中文核心期刊学术论文);参编国际专著一个章节。

SCI期刊论文(仅列出第一作者及通讯作者的SCI期刊论文,*代表通讯作者)

  1.  Wang F#, Fang Q#co-first authors, Wang BB, Yan ZC, Hong J, Bao YM, Kuhn JH, Werren JH, Song QS*, Ye GY* (co-corresponding authors). A novel negative-stranded RNA virus mediates sex ratio in its parasitoid host.PLoS Pathogens, 2017, 13(3): e1006201.

  2.  Teng ZW, Xiong SJ, Xu G, Gan SY, Chen X, Stanley, DW, Yan ZC, Ye GY, Fang Q*. Protein discovery: combined transcriptomic and proteomic analyses of venom from the endoparasitoid Cotesia chilonis (Hymenoptera: Braconidae). Toxins, 2017, 9(4): 135.

  3.  Ye XH, Yang LW, Stanley DW, Li F*, Fang Q*. Two Bombyx mori acetylcholinesterase genes influence motor control and development in different ways. Scientific Reports, 2017, 7: 4985.

  4. Fang Q, Wang BB, Ye XH, Wang F, Ye GY*. Venom of parasitoid, Pteromalus puparum, impairs host humoral antimicrobial activity by decreasing host cecropin and lysozyme gene expression. Toxins, 2016, 8(2): 52.

  5.  Yan ZC#, Fang Q#, Wang L, Liu JD, Zhu Y, Wang F, Li F, Werren JH, Ye GY*. Insights into the venom composition and evolution of an endoparasitoid wasp by combining proteomic and transcriptomic analyses. Scientific Reports, 2016, 6: 19604.

  6.  Xu G, Gu GX, Teng ZW, Wu SF, Huang J, Song QS, Ye GY, Fang Q*. Identification and expression profiles of neuropeptides and their G protein-coupled receptors in the rice stem borer Chilo suppressalis. Scientific Reports, 2016, 6: 28976.

  7.  Zhu Y, Ye XH, Liu Y, Yan ZC, Stanley DW, Ye GY, Fang Q*. A venom gland extracellular chitin-binding-like protein from pupal endoparasitoid wasps, Pteromalus puparum, selectively binds chitin. Toxins, 2015, 7(12): 5098-5113.

  8.  Qi YX, Teng ZW, Gao LF, Wu SF, Huang J, Ye GY, Fang Q*. Transcriptome analysis of an endoparasitoid wasp Cotesia chilonis (Hymenoptera: Braconidae) reveals genes involved in successful parasitism. Archives of Insect Biochemistry and Physiology, 2015, 88(4):203-221.

  9.  Jian-yang Guo, Lu Cheng, Gong-yin Ye, Qi Fang*. Feeding on a begomovirus-infected plant enhances fecundity via increased expression of an insulin-like peptide in the whitefly, MEAM1.Archives of Insect Biochemistry and Physiology, 2014, 85: 164-179.

  10.  Lei Wang#, Qi Fang#, Cen Qian, Fei Wang, Xiao-qiang Yu, Gong-yin Ye*.Inhibition of host cell encapsulation through inhibiting immune gene expression by the parasitic wasp venom calreticulin. Insect Biochemistry and Molecular Biology, 2013, 43: 936-946.

  11.  Lei Wang#, Qi Fang#, Jia-ying Zhu, Fei Wang, Zunnu-raen Akhtar, Gongyin Ye*. Molecular cloning and functional study of calreticulin from a lepidopteran pest, Pieris rapae. Developmental and Comparative Immunology, 2012, 38: 55-65.

  12. Qi Fang, Fei Wang, John A. Gatehouse,Angharad M. R. Gatehouse,Xue-xin Chen, Cui Hu, Gong-yin Ye*. Venom of parasitoid, Pteromalus puparum, suppresses host, Pieris rapae, immune promotion by decreasing host C-type lectin gene expression. PLoS ONE, 2011, 6: e26888.

  13. Qi Fang, Lei Wang, Yang-keng Zhu, David W. Stanley, Xuexin Chen, Cui Hu, Gong-yin Ye*. Pteromalus puparum venom impairs host cellular immune responses by decreasing expression of its scavenger receptor gene. Insect Biochemistry and Molecular Biology, 2011, 41: 852-862.

  14. Qi Fang, Lei Wang, Jia-Ying Zhu, Yan-Min Li, Qi-Sheng Song, David W. Stanley, Zunnu-raen Akhtar and Gong-Yin Ye*. Expression of immune-response genes in lepidopteran host are suppressed by venom from an endoparasitoid, Pteromalus puparum. BMC Genomics, 2010, 11: 484.

  15. Qi Fang, Cheng-hua Huang, Gong-yin Ye*, Hong-wei Yao, Jia-an Cheng and Zunnu-raen Akhtar. Differential fipronil susceptibility and metabolism in two rice stem borers from China. Journal of Economic Entomology, 2008, 101: 1415-1420.


专著

  1. Ye, G.Y., Zhu, J.Y., Zhang, Z., Fang, Q., Cai, J., Hu, C., 2007. Venom from the endoparasitoid Pteromalus puparum (Hymenoptera: Pteromalidae) adversely affects host hemocytes: Differential toxicity and microstructural and ultrastructural changes in plasmatocytes and granular cells. In Rivers D., Yoder J. (Eds.): Recent Advances in the Biochemistry, Toxicity, and Mode of Action of Parasitic Wasp Venoms. Research Signpost, pp. 115-127.


发明专利

  1. 叶恭银, 王磊, 方琦, 王飞, 蝶蛹金小蜂毒液Kazal类丝氨酸蛋白酶抑制剂多肽及应用,2014.03,中国,ZL201210208083.4.

  2. 叶恭银, 王磊, 方琦, 王飞, 朱洋铿. 蝶蛹金小蜂毒液丝氨酸蛋白酶抑制剂Pp-PI多肽及应用,2013.02,中国,ZL201110201939.0.

  3. 慎小晶, 叶恭银, 方琦, 朱家颖, 成雄鹰, 胡萃, 蝶蛹金小蜂抗菌蛋白Pp-AP5及其编码的核苷酸序列, 专利号: ZL200810120143.0.


主要学术兼职

  1. 担任国际学术期刊《Frontiers in Physiology(Invertebrate Physiology Section) Review Editor

  2. 浙江省植物保护学会青年工作委员会副主任委员;

  3. SCI期刊《Transgenic Research》、《Archives of Insect Biochemistry and Physiology》、《Journal of Asia-Pacific Entomology》、《Frontiers in Experimental Endocrinology》等SCI期刊审稿人。