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方卫国 教授

杭州市西湖区余杭塘路866

邮编:310058

电话:0571-88206665(办公室)

E-mail: wfang1@zju.edu.cn

Website: http://mypage.zju.edu.cn/weiguofang

方卫国

简历

方卫国,博士,青年千人计划学者,博士生导师,浙江大学生科院和浙江大学“昆虫学科”双聘兼职教授

  

   2003毕业于西南农业大学生物技术中心并获得博士学位。以博士后和Research Associate身份,先后在加拿大Brock University年美国马里兰大学(College Park)从事合作研究近8年2012年加盟浙江大学,同年聘为美国马里兰大学(College Park)昆虫学系客座教授。

研究领域

      

    我们的研究集中于病原真菌寄主互作机理和形成这种互作的进化机制。目前已经发现了1000 种真菌能感染昆虫,但它们在真菌系统进化树上呈随机分布,感染昆虫的能力和亲缘关系无相关性,是研究真菌毒力进化的一类独特材料。罗伯茨绿僵菌的致病方式 具有较广的代表性,在昆虫体表,其致病方式与一些植物病原真菌的类似(如产生附着孢等),进入昆虫血腔之后,它抵御寄主天然免疫的机制与人类和哺乳动物病 原真菌类似(抵御免疫细胞吞噬和抗菌肽的攻击等)。我们以罗伯茨绿僵菌为材料,采用功能基因组学、分子生物学和生物信息学等手段,深入研究它与寄主的互作 机理,并探究形成这些互作机理的进化机制。

一些昆虫病原真菌已被开发成真菌杀虫剂,用于防治农林害虫、草原害虫、卫生害虫和它们传播的重大虫媒疾病(疟疾和登革热等)。在研究病原真菌与昆虫互作机理的基础上, 创建高效安全的真菌杀虫剂。

教学工作

《微生物学》:《Brook Biology of Microorganisms13th Edition, edited by Michael Madigan et al., Pearson International

在读研究生9名,其中博士研究生4名。

学术兼职

1. 中国菌物学会 理事

2. 中国植物保护学会青年理事会理事

3. 浙江省微生物学会理事

 

奖励荣誉

1. “国家青年千人计划2012 2批)

2. 浙江省杰出青年基金(2013年)

发表论著 * 通讯作者)

在国内期刊发表论文50多篇,其中在Science等期刊发表SCI论文30多篇,影响因子(IF)共计近190分。2012年回国后,以通讯作者身份,在PNASPLoS PathogensEnviron Microbiol等期刊发表论文7篇。近5SCI他引600多次。“Faculty of 1000”推荐论文3篇。美国微生物学会“This Week in Microbiology”专题报道论文2篇。

1.       Chen X, Xu C, Qian Y, Liu R, Zhang Q, Zeng G, Zhang X, Zhao H, Fang W*.MAPK cascade-mediated regulation of pathogenicity, conidiation and tolerance to abiotic stresses in the entomopathogenic fungus Metarhizium robertsii. Environ Microbiol. 2015 Dec 30. doi: 10.1111/1462-2920.13198.

2.       Zhao H, Lovett B R, Fang W*. Genetically Engineering Entomopathogenic Fungi. Advance in Genetics (in press) (Invited review), 2016

3.       Fang, W*, Lu, HL., King, GF. & St.Leger, RJ. Construction of a Hypervirulent and Specific Mycoinsecticide for Locust Control. Sci. Rep. 4, 7345; DOI:10.1038/srep07345 (2014).

4.       Xu C, Zhang X, Qian Y, Chen X, Liu R, Zeng G, Zhao H, Fang W*. A high-throughput gene disruption methodology for the entomopathogenic fungus Metarhizium robertsii. PLoS ONE 2014 Sep 15;9(9):e107657

5.       Liao X*, O'Brien TR, Fang W, St Leger RJ.  The plant beneficial effects of Metarhizium species correlate with their association with roots. Appl Microbiol Biotechnol. 2014 May 8.

6.       Zhao H, Xu C, Lu HL,  Chen X, St.Leger RJS, Fang W* Host-to-pathogen gene transfer facilitated infection of insects by a pathogenic fungus. PLoS Pathogens  2014 Apr 10;10(4):e1004009. doi: 10.1371/journal.ppat.1004009.   Digested by Microbe World (This Week in Microbiology): http://www.microbeworld.org/podcasts/this-week-in-microbiology/archives/1677-twim-78-a-bacterium-grows-in-brooklyn

7.       Shi W*, Guo Y, Xu C, Tan S, Miao J,Feng Y, Zhao H, St. Leger RJ, Fang W* Unveiling the mechanism for preventing migratory locust’s swarm behavior by microsporidian parasites Proc Natl Acad Sci USA 2014 Jan 28;111(4):1343-8. Faculty of 1000 Highlighted by Science: http://news.sciencemag.org/plants-animals/2014/01/gut-parasite-may-keep-locusts-swarming. Digested by Microbe World (This Week in Microbiology): http://www.microbeworld.org/index.php?option=com_content&view=category&layout=blog&id=107&Itemid=275

8.       Liao X, LU HL, Fang W, St.Leger RJ. Overexpression of a Metarhizium robertsii HSP25 gene increases thermotolerance and survival in soil. Appl Microbiol Biotechnol. 2014 Jan;98(2):777-83.

9.       Liao X, Fang W, Lin L, Lu HL, St.leger RJ. Metarhizium robertsii Produces an Extracellular Invertase (MrINV) That Plays a Pivotal Role in Rhizospheric Interactions and Root Colonization.  PLoS One. 2013 Oct 21;8(10):e78118. doi: 10.1371/journal.pone.0078118.

10.   Fang W, St. Leger RJ. Enhanced UV Resistance and Improved Killing of Malaria Mosquitoes by Photolyase Transgenic Entomopathogenic Fungi. PLoS One 2012;7(8):e43069

11.   Fang W, Azimzadeh P, St Leger RJ. Strain improvement of fungal insecticides for controlling insect pests and vector-borne diseases. Curr Opin Microbiol. 201215:232-238.

12.   Lin L, Fang W, Liao X, Wang F, Wei D, St Leger RJ. The MrCYP52 cytochrome P450monoxygenase gene of Metarhizium robertsii is important for utilizing insect epicuticular hydrocarbons. PLoS One. 2011;6(12):e28984.

13.   Wang S, Fang W, Wang C, St. Leger RJ. Insertion of an esterase gene into a specific locust pathogen (Metarhizium acridum) enables it to infect caterpillars. PLoS Pathog. 2011 7(6):e1002097.

14.   Fang W, Vega-Rodríguez J, Ghosh AK, Jacobs-Lorena M, Kang A, St Leger RJ. Development of transgenic fungi that kill human malaria parasites in mosquitoes. Science. 2011 331(6020):1074-7. Faculty of 1000

15.   Gao Q, Jin K, Ying SH, Zhang Y, Xiao G, Shang Y, Duan Z, Hu X, Xie XQ, Zhou G, Peng G, Luo Z, Huang W, Wang B, Fang W, Wang S, Zhong Y, Ma LJ, St Leger RJ, Zhao GP, Pei Y, Feng MG, Xia Y, Wang C. Genome sequencing and comparative transcriptomics of the model entomopathogenic fungi Metarhizium anisopliae and M. acridum. PLoS Genet. 2011 6;7(1):e1001264. Faculty of 1000

16.   Pava-Ripoll M, Angelini C, Fang W, Wang S, Posada FJ, St Leger R. The rhizosphere-competent entomopathogen Metarhizium anisopliae expresses a specific subset of genes in plant root exudate. Microbiology. 2011 157:47-55.

17.   Fang W, St Leger RJ. Mrt, a gene unique to fungi, encodes an oligosaccharide transporter and facilitates rhizosphere competency in Metarhizium robertsii. Plant Physiol. 2010 154(3):1549-57.

18.   Fang W, Fernandes EK, Roberts DW, Bidochka MJ, St Leger RJ. A laccase exclusively expressed by Metarhizium anisopliae during isotropic growth is involved in pigmentation, tolerance to abiotic stresses and virulence. Fungal Genet Biol. 2010 47(7):602-7.

19.   Fang W, St Leger RJ. RNA binding proteins mediate the ability of a fungus to adapt to the cold. Environ Microbiol. 2010 12(3):810-20.

20.   Jin K, Zhang Y, Fang W, Luo Z, Zhou Y, Pei Y. Carboxylate transporter gene JEN1 from the entomopathogenic fungus Beauveria bassiana is involved in conidiation and virulence. Appl Environ Microbiol. 2010 76(1):254-63.

21.   Fang W, Feng J, Fan Y, Zhang Y, Bidochka MJ, Leger RJ, Pei Y. Expressing a fusion protein with protease and chitinase activities increases the virulence of the insect pathogen Beauveria bassiana. J Invertebr Pathol. 2009 102(2):155-9.

22.   Wang S, Leclerque A, Pava-Ripoll M, Fang W, St Leger RJ. Comparative genomics using microarrays reveals divergence and loss of virulence-associated genes in host-specific strains of the insect pathogen Metarhizium anisopliae. Eukaryot Cell. 2009 8(6):888-98.

23.   Zhang Y, Zhao J, Fang W, Zhang J, Luo Z, Zhang M, Fan Y, Pei Y. Mitogen-activated protein kinase hog1 in the entomopathogenic fungus Beauveria bassiana regulates environmental stress responses and virulence to insects. Appl Environ Microbiol. 2009 75(11):3787-95.

24.   Fang W, Pava-ripoll M, Wang S, St Leger R. Protein kinase A regulates production of virulence determinants by the entomopathogenic fungus, Metarhizium anisopliae. Fungal Genet Biol. 2009 46(3):277-85.

25.   Fang W, Scully LR, Zhang L, Pei Y, Bidochka MJ. Implication of a regulator of G protein signalling (BbRGS1) in conidiation and conidial thermotolerance of the insect pathogenic fungus Beauveria bassiana. FEMS Microbiol Lett. 2008 279(2):146-56.

26.   Liao XG, Fang WG, Zhang YJ, Fan YH, Wu XW, Zhou Q, Pei Y. Characterization of a highly active promoter, PBbgpd, in Beauveria bassiana. Curr Microbiol. 2008 57 :121-6.

27.   Fan Y, Fang W, Xiao Y, Yang X, Zhang Y, Bidochka MJ, Pei Y. Directed evolution for increased chitinase activity. Appl Microbiol Biotechnol. 2007 76(1):135-9.

28.   Fang W, Pei Y, Bidochka MJ. A regulator of a G protein signalling (RGS) gene, cag8, from the insect-pathogenic fungus Metarhizium anisopliae is involved in conidiation, virulence and hydrophobin synthesis. Microbiology. 2007 153:1017-25.

29.   Fan Y*, Fang W*, Guo S, Pei X, Zhang Y, Xiao Y, Li D, Jin K, Bidochka MJ, Pei Y. Increased insect virulence in Beauveria bassiana strains overexpressing an engineered chitinase. Appl Environ Microbiol. 2007 73(1):295-302. (*: contribute equally)

30.   Fang W, Bidochka MJ. Expression of genes involved in germination, conidiogenesis and pathogenesis in Metarhizium anisopliae using quantitative real-time RT-PCR. Mycol Res. 2006 110:1165-71.

31.   Fang W, Pei Y, Bidochka MJ. Transformation of Metarhizium anisopliae mediated by Agrobacterium tumefaciens. Can J Microbiol. 2006 52(7):623-6

32.   Yang X, Li J, Wang X, Fang W, Bidochka MJ, She R, Xiao Y, Pei Y. Psc-AFP, an antifungal protein with trypsin inhibitor activity from Psoralea corylifolia seeds. Peptides. 2006 27(7):1726-31.

33.   Fang W, Leng B, Xiao Y, Jin K, Ma J, Fan Y, Feng J, Yang X, Zhang Y, Pei Y. Cloning of Beauveria bassiana chitinase gene Bbchit1 and its application to improve fungal strain virulence. Appl Environ Microbiol. 2005 71(1):363-70.

34.   Fang W, Zhang Y, Yang X, Zheng X, Duan H, Li Y, Pei Y. Agrobacterium tumefaciens-mediated transformation of Beauveria bassiana using an herbicide resistance gene as a selection marker. J Invertebr Pathol. 2004 Jan;85(1):18-24.